Study on the Relationship Between the Antipyretic Activity and Chemical Quality Control Components of Reduning Injection

OBJECTIVE  To explore the relationship between the antipyretic activity and chemical quality control components of Reduning Injection(RDN).METHODS  RDN was administered on lipopolysaccharide(LPS)-induced fever rats to observe the antipyretic effect and detect the level of PGE₂ in hypothalamus tissue. LPS-induced RAW 264.7 cells were replicated and cyclooxygenase(COX)-2 enzyme assay were performed to evaluate the activity of RDN and its chemical components against PGE ₂ and COX-2.RESULTS  RDN could obviously reduce the temperature rise in fever rats, and PGE₂ level in hypothalamus tissues. RDN could also inhibit the release of PGE₂ and the activity of COX-2, with IC₅₀ of 27.07 μg/mL, 418.6 ng/mL, respectively. Meanwhile, the level of PGE₂ was reduced by caffeic acid, neochlorogenci acid and isochlorogenic acid A, isochlorogenic acid B, while the activity of COX-2 was inhibited by chlorogenic acid, crytochlorogenic acid, caffeic acid and isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C.CONCLUSION  RDN shows significant antipyretic effect via inhibiting the activity of COX-2 and reducing the release of PGE₂, which was related to caffeolyquinic acids in chemical quality control indexes.