鸦胆子饮片及药渣的抗肿瘤活性比较及其鸦胆子苦醇和鸦胆子苦素A的含量测定

严国俊; 韩露; 谢辉; 苏联麟; 马晓斐; 张倩; 陆兔林; 毛春芹

鸦胆子饮片及药渣的抗肿瘤活性比较及其鸦胆子苦醇和鸦胆子苦素A的含量测定

南京中医药大学药学院，江苏南京 210023

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出版历程
- 刊出日期: 2019-11-10

Investigation of Antitumor Activity of Brucea Javanica Dregs by RTCA and Determination of Brusatol and Bruceine A from Brucea Javanica Pieces and Dregs

摘要

摘要: 目的比较鸦胆子饮片及提油后药渣的抗肿瘤活性，并比较两者的苦木内酯类成分鸦胆子苦醇及鸦胆子苦素A的含量。方法采用实时电子细胞分析技术（RTCA）考察鸦胆子饮片及药渣对A549细胞的抑制效应，并采用高效液相色谱法同时测定两者的鸦胆子苦醇及鸦胆子苦素A的含量。结果鸦胆子饮片及药渣对A549细胞都具有一定的抑制效应，饮片和药渣的IC₅₀值分别为4.576~7.256 mg/mL和4.115~9.766 mg/mL，鸦胆子饮片和鸦胆子药渣中鸦胆子苦醇及鸦胆子苦素A的总含量分别为0.113 9%~0.143 5%和0.110 0%~0.135 9%。结论鸦胆子饮片及药渣都具有一定的抗肿瘤活性，药渣中的抗肿瘤活性成分鸦胆子苦醇及鸦胆子苦素A的含量与饮片比较，没有显著差异，鸦胆子提油后的药渣可针对苦木内酯类成分进行深入研究开发和资源综合利用。
- 鸦胆子 /
- 实时电子细胞分析技术 /
- 鸦胆子苦醇 /
- 鸦胆子苦素A /
- 含量测定
Abstract: OBJECTIVE To investigate the antitumor activity of Brucea javanica pieces and the extracted dregs， and to compare the contents of Brusatol and Bruceine A. METHODS The inhibitory effect of Brucea javanica pieces and dregs on A549 cells was investigated by Real-time cell-based assay (RTCA)， and the contents of Brusatol and Bruceine A were determined by HPLC simultaneously. RESULTS The Brucea javanica pieces and dregs both showed certain inhibitory effects on A549 cells and the IC₅₀ values were 4.576-7.256 mg/mL and 4.115-9.766 mg/mL. The total content of Brusatol and Bruceine A in Brucea javanica pieces and the extracted dregs were 0.113 9%-0.143 5% and 0.110 0%-0.135 9%. CONCLUSION The pieces and dregs of Brucea javanica have certain antitumor activity， and there is no significant difference in the contents of anti-tumor components between the dregs and the pieces. The dregs of Brucea javanica can be researched and developed in depth centering on nigakilactone. It also can promote the comprehensive use of Chinese medicinal resources.
- Brucea javanica /
- RTCA /
- Brusatol /
- Bruceine A /
- content determination

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