糖脂清对糖尿病大鼠海马内质网应激相关因子GRP78、CHOP、Caspase-12表达的影响

王旭; 邵鑫; 许海燕; 叶丽芳; 徐奚如; 杨晓青

糖脂清对糖尿病大鼠海马内质网应激相关因子GRP78、CHOP、Caspase-12表达的影响

1.
南京中医药大学附属医院，江苏南京 210029
2.
南京中医药大学第三附属医院，江苏南京 210001
3.
徐州医科大学基础医学院，江苏徐州 221004

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出版历程
- 刊出日期: 2019-01-10

Effects Tangzhiqing on the Expression of Endoplasmic Reticulum Stress Related Factors GRP78， CHOP and Caspase-12 in the Hippocampus of Diabetic Rats

1.
Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China
2.
The Third Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210001, China
3.
Basic Medical College, Xuzhou Medical University, Xuzhou, 221004, China

摘要

摘要: 目的通过观察糖脂清对糖尿病大鼠海马组织的内质网应激相关因子GPR78、CHOP、Caspase-12的影响，探讨其在改善糖尿病大鼠认知功能的作用机制。方法采用高糖高脂饲料联合小剂量链脲佐菌素腹腔注射复制2型糖尿病大鼠模型，并给予糖脂清高、中、低剂量组干预8周后，采用ELISA法检测血清空腹葡萄糖（FPG）、空腹胰岛素（FINS），计算稳态模型的胰岛素抵抗指数（HOMA-IR）、胰岛素敏感指数（ISI）；qPCR和Western blot法检测海马组织内质网应激相关因子GPR78、CHOP、Caspase-12的mRNA及蛋白表达水平。结果糖脂清各剂量组均能够显著降低FPG及HOMA-IR（ P <0.01），除低剂量组外，糖脂清均可显著提高ISI（ P <0.01），3组FINS水平无显著性差异（ P >0.05）。与模型组比较，糖脂清低、中、高3个剂量组GRP78 mRNA和蛋白相对表达均显著增加，CHOP、Caspase-12 mRNA表达均减少（ P <0.01），中、高剂量组CHOP、Caspase-12蛋白表达显著减少（ P <0.01）。结论糖脂清能够控制血糖，改善胰岛素抵抗，增加胰岛素敏感性，同时能够调节海马内质网应激相关因子GRP78、CHOP、Caspase-12 mRNA和蛋白的表达。
- 糖脂清 /
- 2型糖尿病 /
- 内质网应激 /
- GRP78 /
- CHOP /
- Caspase-12
Abstract: OBJECTIVE To observe the effect of Tangzhiqing on the endoplasmic reticulum stress related factors GPR78， CHOP and Caspase-12 in hippocampus of diabetic rats， and to explore its mechanism of improving cognitive function in diabetic rats. METHODS The model of type 2 diabetes mellitus rats was established by intraperitoneal injection of low-dose of streptozotocin combined with high-sugar and high-fat diet， After 8 weeks of intervention with high， medium and low-dose of Tangzhiqing， serum fasting glucose (FPG) and fasting insulin (FINS) were detected by ELISA， the index of insulin resistance (HOMA-IR) and insulin sensitivity index (ISI) were calculated; the expression levels of endoplasmic reticulum stress related genes GPR78， CHOP and Caspase-12 mRNA and protein in hippocampus were detected by qPCR and Western blot assay. RESULTS Different dosages of Tangzhiqing significantly decreased FPG and HOMA-IR ( P <0.01); except the low dose group， Tangzhiqing significantly improved ISI ( P <0.01); no significant difference existed in FPG level among the 3 groups ( P >0.05). Compared to the model group， the relative expression of GRP78 mRNA and protein in the 3 groups of Tangzhiqing increased significantly， and the expression of CHOP and Caspase-12 mRNA in these 3 groups decreased ( P <0.01). CHOP and Caspase-12 in middle and high dose group decreased significantly ( P <0.01). CONCLUSION Tangzhiqing can control blood sugar， improve insulin resistance， increase insulin sensitivity and regulate the expression of GRP78， CHOP， Caspase-12 in hippocampus.
- Tangzhiqing /
- type 2 diabete /
- endoplasmic reticulum stress /
- GRP78 /
- CHOP /
- Caspase-12

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参考文献(16)

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