桂枝茯苓胶囊对EM模型大鼠异位内膜细胞凋亡、VEGF及血清雌孕激素的影响

肖新春; 李海燕; 李昕芹

桂枝茯苓胶囊对EM模型大鼠异位内膜细胞凋亡、VEGF及血清雌孕激素的影响

1.
陕西中医药大学附属医院，陕西咸阳 712046
2.
渭南市妇幼保健院妇科，陕西渭南 714000
3.
山西省中医院妇科，山西太原 030000

计量
- 文章访问数: 607
- HTML全文浏览量: 5
- PDF下载量: 650
- 被引次数: 0
出版历程
- 刊出日期: 2019-01-10

Effects of Guizhi Fuling Capsule on Apoptosis of Heterotopic Endometrial Cells， VEGF and Estrogen and Progesterone in the Serum of Rat EM Model

1.
The Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine, Xianyang, 712046, China
2.
Department of Gynecology, Weinan City Maternal and Child Health Hospital, Weinan, 714000, China
3.
Department of Gynecology, Shanxi Provincial Hospital of Traditional Chinese Medicine, Taiyuan, 030000, China

摘要

摘要: 目的以自体移植法建立EM大鼠模型，观察桂枝茯苓胶囊对EM大鼠异位内膜细胞凋亡、VEGF及血清雌、孕激素含量的影响，探讨其治疗EM的效应机制。方法采用自体移植法建立大鼠子宫内膜异位症模型，术后4周将造模成功大鼠随机分为模型组、米非司酮组、散结镇痛胶囊组、桂枝茯苓胶囊高、中、低剂量组，药物干预4周后进行大鼠异位内膜细胞凋亡TUNEL法检测，检测大鼠血清中雌、孕激素的含量，免疫组化法检测Caspase-7、VEGF、survivin表达。结果桂枝茯苓高剂量组异位内膜细胞凋亡率显著高于模型组，桂枝茯苓高剂量组及米非司酮组异位内膜Caspase-7表达均较模型组显著升高，VEGF、survivin表达及血清雌、孕激素含量显著降低。结论桂枝茯苓胶囊治疗EMS的疗效机制，可能与诱导异位内膜细胞凋亡、下调survivin的高表达、降低VEGF表达及调节血清中雌孕激素水平密切相关。
- 桂枝茯苓胶囊 /
- 子宫内膜异位症 /
- 细胞凋亡 /
- survivin /
- VEGF
Abstract: OBJECTIVE To observe the effect of VEGF， estrogen and progesterone in serum on ectopic endometrial cell apoptosis in EMS rats and explore the mechanism of Guizhi Fuling Capsule. METHODS Establish rat endometriosis model by autologous transplantation methods， Four weeks after surgery， The rats were randomly divided into model group， mifepristone group， Sanjie analgesic capsule group， Guizhi Fuling pill high， medium and low dose group，with 15 rats per group. Four weeks after the drug intervention， the ectopic endometrial cell apoptosis was detected by TUNEL method The contents of estrogen and progesterone in serum were measured，The expression of caspase-7， VEGF and survivin was detected by immunohistochemistry. RESULTS The apoptotic rate of ectopic endometrial cells in Guizhi Fuling high dose group was significantly higher than model group，The expression of caspase-7 in ectopic endometrium of Guizhi Fuling high dose group and mifepristone group was significantly higher than model group，VEGF， survivin expression and the content of estrogen and progesterone in serum were significantly decreased. CONCLUSION The mechanism of the treatment of EMT by Guizhi Fuling Capsule， may be closely related to induced ectopic endometrial cell apoptosis and decreased endometrial VEGF expression and down-regulates the high expression of survivin and regulated levels of estrogen and progesterone in serum.
- Guizhi Fuling Capsule /
- endometriosis /
- apoptosis /
- survivin /
- VEGF

HTML全文

参考文献(12)

[1]	李雅男，王丹波，陈英汉，等.不同类型子宫内膜异位症临床特点及意义分析[J]. 实用妇产科杂志，2015，31(1):34-36.
[2]	VERNON M， WILSON A. Study on the surgical induction of endometriosis in the rats[J]. Fertil Steril，1985，44:782-786.
[3]	施新猷.现代医学实验动物学[M].北京:人民军医出版社，2002:10.
[4]	ZULFIKAROGLU E，KILIC S，ISLIMYE M， et al．Efficacy of antitumor necrosis factor therapy on endometriosis in an experimental rat model[J]．Arch Gynecol Obstet，2011，283（4）：799-804.
[5]	齐之迎，尹利荣，马洪达，等.芳香化酶抑制剂对大鼠子宫内膜异位灶中生成素及caspase-3的影响[J].天津医药，2011，39(3):260-263.
[6]	FERRERO S， CAMERINI G， SERACCHIOLI R， et al. Letrozole combined with norethisterone acetate compared with norethisterone acetate alone in the treatment of pain symptom caused by endometriosis[J]. Hum Reprod， 2009，24:3033-3041.
[7]	梁铮，吴瑞瑾.细胞凋亡与子宫内膜异位症[J].国际妇产科学杂志，2009，36(5):356-359.
[8]	杨涛，费振海，钟兴明.Caspase家族与细胞凋亡的研究进展[J].浙江医学，2018，40（18）:2083-2087.
[9]	XU JY， WANG SF，ZHU WJ，et al. Expression of survivin gene among human normal endometrium， atypical hyperplasia of endometrium and endometrial carcinoma[J].J Reproduct Contracept，2010，21(3):129-135.
[10]	ROSENSTEIN JM， MANI N，SILVEMAN WF， et al. Patterns of brain angiogenesis after vascular endothelial growth factor administration in vitro and in vivo [J]. Proc Natl Acad Sci USA，1998，95(12): 7086-7091.
[11]	肖新春，崔晓萍.消异方对子宫内膜异位症大鼠血清E₂、P含量的影响[J].中国中医药科技，2013，20（5）:465-466.
[12]	胡妮娜，赵闯，张金琦，等.桂枝茯苓丸治疗子宫内膜异位症的临床及机制研究进展[J].中医药学报，2017，45(4):104-107.