黄芩苷对辐射诱导的小鼠急性肺损伤保护作用机制研究

葛永亮; 陶金华; 吴木军

黄芩苷对辐射诱导的小鼠急性肺损伤保护作用机制研究

1.
南通市通州区人民医院影像科，江苏南通 226000
2.
南通大学药学院，江苏南通 226001

计量
- 文章访问数: 1080
- HTML全文浏览量: 8
- PDF下载量: 1006
- 被引次数: 0
出版历程
- 刊出日期: 2017-11-10

Effect of Baicalin on Radiation-Induced Acute Lung Injury in Mice Through Antioxidant and Anti-Inflammatory Properties

1.
Department of Radiology, Tongzhou District People's Hospital, Nantong, 226000, China
2.
School of Pharmacy, Nantong University, Nantong, 226001, China

摘要

摘要: 目的探讨黄芩苷对辐射诱导小鼠的急性肺损伤保护作用。方法 X-线复制小鼠的急性肺损动物模型。将50只ICR小鼠随机分为空白对照组、X-线组、X-线组+地塞米松组（5mg/kg）、X-线组+黄芩苷组（20、40mg/kg）。观察各组肺组织病理学改变，测量肺湿/干质量比，支气管肺泡灌洗液中性粒细胞百分比、肺组织中超氧化物歧化酶（SOD）、丙二醛（MDA）、炎症因子白介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）含量及肺组织中p-NFκBp65、p-IκBα蛋白表达。结果黄芩苷组（20、40mg/kg）可有效减轻辐射所致肺组织病理学变化，能降低肺泡灌洗液中炎症细胞的数目，能显著降低肺湿/干质量比重，能提高SOD水平，并能降低MDA水平，降低p-NFκBP65和p-IκBα蛋白的表达。结论黄芩苷可减轻辐射所致急性肺组织损伤，对辐射诱导的急性肺损伤有保护作用。
- 黄芩苷 /
- 辐射 /
- 急性肺损伤 /
- NF-κBp65
Abstract: OBJECTIVE The current study was designed to evaluate the protective effects of baicalin on radiation-induced acute lung injury in mice. METHODS 50 ICR mice were divided into five groups randomly， namely， control group， X-ray group， X-ray+dexamethasone group (5mg/kg)， X-ray+baicalin group (20， 40 mg/kg)， with 8 in each group. Pathological changes in the lung tissues were observed. The ratio of lung wet/dry weight was also recorded. Besides， the level of neutrophil in bronchoalveolar lavage fluid (BALF) was detected to evaluate inflammatory cell infiltration. Moreover， the expressions of MDA， IL-6， TNF-α， p-NFκBp65 and p-IκBα， as well as the activity of SOD， were examined in lung. RESULTS Baicalin (20， 40 mg/kg) could effectively improve the radiation-induced lung pathological changes and reduce the number of inflammatory cells in BALF. The ratio of lung wet/dry weight was also decreased with the treatment of baicalin (20， 40 mg/kg). In addition， the activity of SOD was recovered and the end product of lipid peroxidation MDA was reduced meanwhile. The expressions of p-NFκBp65 and p-IκBα was extensively down-regulated with baicalin (20， 40 mg/kg) preconditioning， taking the expressions of NFκBp65 and IκBα as inner controls. CONCLUSION Baicalin could alleviate radiation-induced acute lung injury due to its antioxidant and anti-inflammatory properties.
- baicalin /
- radiation /
- acute lung injury /
- NF-κBp65

HTML全文

参考文献(19)

[1]	ALIYU UM， JIMOH AO， YUNUSA A， et al. Pattern of cancers treated with radiotherapy in uduth sokoto: A new center experience[J]. J Clin Diag Res， 2015， 9(8):1-4.
[2]	WATANABE H， SUGA A， TSUCHIHASHI Y， et al. Clinical study of radiation pneumonitis over 10 years[J]. Nihon Kyōbu Shikkan Gakkai Zasshi， 1995， 33(4): 384-388.
[3]	王跃珍．放射性肺炎研究进展[J]．中国肿瘤，2007， 16（1）：39-43．
[4]	WANG YZ. Research progress of radiation pneumonitis[J]. Bull Chin Cancer， 2007， 16(1): 39-43.
[5]	陈璐，李志斌，张德明，等．放射性肺炎研究进展[J]．中国现代医学杂志，2010， 20（2）：281-284．
[6]	CHEN L， LI ZB， ZHANG DM， et al. Progress of radiation pneumonitis research[J]. China J Mod Med， 2010， 20(2): 281-284.
[7]	DONG SJ， ZHONG YQ， LU WT， et al. Baicalin inhibits Lipopolysaccharide-Induced inflammation through signaling NF-κB pathway in HBE16 airway epithelial cells[J]. Inflammation， 2015， 38(4): 1493-1501.
[8]	WEN YF， ZHAO JQ， BHADAURIA M， et al. Baicalin prevents Cadmium induced hepatic cytotoxicity， oxidative stress and histomorphometric alterations[J]. Exp Toxicol Pathol， 2013， 65(1/2): 189-196.
[9]	刘煜德，吴伟，王嵩，等．肺炎衣原体对主动脉粥样硬化斑块的形成及稳定性的影响及黄芩苷的干预作用[J]．时珍国医国药，2006， 17（12）：2377-2379．
[10]	LIU YD， WU W， WANG S， et al. The influence of CPn infection on the formation and stability of atherosclerosis plaque and the intervention of baicalin[J]. Lishizhen Med Mater Med Res， 2006， 17(12): 2377-2379.
[11]	窦永起，杨明会，林明雄，等．放射性肺损伤中医证候学特点及其演变规律的研究[J]．中华中医药杂志，2009，24（6）：706-708．
[12]	DOU YQ， YANG MH， LIN MX， et al. Experimental study on TCM symptomatological and developmental characteristics of rats with radiation-induced lung injury[J]. China J Tradit Chin Med Pharm， 2009，24(6): 706-708.
[13]	MALAVIYA R， GOW AJ， FRANCIS M， et al. Radiation-Induced lung injury and inflammation in mice: role of inducible nitric oxide synthase and surfactant protein D[J]. Toxicol Sci， 2015， 144(1): 27-38.
[14]	KUO MY， LIAO MF， CHEN FL， et al. Luteolin attenuates the pulmonary inflammatory response involves abilities of antioxidation and inhibition of MAPK and NF kappa B pathways in mice with endotoxin-induced acute lung injury[J]. Food Chem Toxicol， 2011， 49(10): 2660-2666.
[15]	LI YC， YEH CH， YANG ML， et al. Luteolin suppresses inflammatory mediator expression by blocking the Akt/NF kappa B pathway in acute lung injury induced by lipopolysaccharide in mice[J]. Evid Based Complement Alternat Med， 2012: 383608.
[16]	JANG SS， KIM HG， LEE JS， et al. Melatonin reduces X-ray radiation-induced lung injury in mice by modulating oxidative stress and cytokine expression[J]. Int J Radiat Biol， 2013， 89(2): 97-105.
[17]	EPPERLY MW， DEFILIPPI S， SIKORA C， et al. Intratracheal injection of Manganese superoxide dismutase(MnSOD)plasmid/liposomes protects normal lung but not orthotopic tumors from irradiation[J]. Gene Ther， 2000， 7(12): 1011-1018.
[18]	WANG J， XU HW， LI BS， et al. Preliminary study of protective effects of flavonoids against radiation-induced lung injury in mice[J]. Asian Pac J Cancer Prev， 2012， 13(12): 6441-6446.
[19]	TAHAMTAN R， MONFARED AS， TAHAMTANI YA， et al. Radioprotective effect of melatonin on Radiation-Induced lung injury and lipid peroxidation in rats[J]. Cell J， 2015， 17(1): 111-120.