养心2号方对慢性心力衰竭大鼠心肌组织ATP及MMP的影响

朱云仙; 张斌霞; 潘涛

养心2号方对慢性心力衰竭大鼠心肌组织ATP及MMP的影响

南京中医药大学第一临床医学院，江苏南京 210023;2常州市中医院心内科，江苏常州 213003

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出版历程
- 刊出日期: 2016-05-10

The Effect of Yangxin2 Fang on the Myocardial ATP and MMP in Rats with Chronic Heart Failure

The First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing, 210023, China

摘要

摘要: 目的研究养心2号方对慢性心力衰竭（CHF）大鼠心肌组织能量代谢的影响，探讨其治疗CHF的作用机制。方法将45只清洁级SD大鼠随机分为正常对照组（A组，n=6）及CHF组（n=39），CHF组采用连续28d皮下注射异丙肾上腺素(ISO)方法建立慢性心力衰竭模型。造模成功后，将存活的CHF组大鼠随机分为模型组（B组，n=6）、卡维地洛组（C组，n=6）及养心2号组（D组，n=6）。A组、B组大鼠予蒸馏水灌胃，C组、D组分别予卡维地洛、养心2号方灌胃。各组均连续灌胃28d后，水合氯醛麻醉下取心肌组织，分别测定组织中三磷酸腺苷（ATP）浓度及线粒体膜电位（MMP）水平。结果 ①ATP浓度变化：B组ATP浓度下降，与A组比较差异有统计学意义（P＜0.05）；D组ATP浓度升高，与B组及C组比较，差异有显著统计学意义（P＜0.01）；②MMP水平变化：B组MMP水平显著下降，与A组比较差异有显著统计学意义（P＜0.01）；D组、C组MMP水平均明显升高，与B组比较差异有显著统计学意义（P＜0.01）。结论养心2号方能升高慢性心衰模型大鼠心肌组织的ATP浓度，同时升高MMP水平，具有明显改善心肌组织能量代谢的作用，该作用可能是养心2号方治疗CHF的作用机制之一。
- 慢性心力衰竭 /
- 养心2号方 /
- 三磷酸腺苷 /
- 线粒体膜电位
Abstract: OBJECTIVE To study the effect of Yangxin2 Fang on the myocardial energy metabolism in rats with chronic heart failure（CHF）， and to explore the mechanism of Yangxin2 Fang for the treatment of CHF. METHODS 45 male clean grade SD rats were divided into two groups， normal group（group A，n=6） and CHF group（n=39）.The CHF group was injected hypodermic with ISO for 28 consecutive days. After the model was successfully established in CHF， the survival rats were randomly divided into three groups: model group （group B）， Carvedilol group （group C）and Yangxin2 Fang group（group D）.Group C and group D were received gastric gavage of Carvedilol and Yangxin2 Fang respectively. Group A and group B were received gastric gavage of distilled water. After 28 days of dosing， the levels of myocardial adenosine triphosphate （ATP）and mitochondrial membrane potential （MMP） were tested. RESULTS ①the change of ATP level: Compared with the group A， the level of myocardial ATP was decreased in group B（P＜0.05）; Compared with the group B and C， the level of myocardial ATP was increased in group D（P＜0.01）.Compared with group C， the level of myocardial ATP was increased in group D（P＜0.01）. ②the change of MMP levels： Compared with group A， the levels of MMP was decreased in group B（P＜0.01） Compared with the group B， the levels of myocardial MMP were increased in group C and D.（P＜0.01）. CONCLUSION Yangxin2 Fang could increase the levels of myocardial ATP and MMP to improve myocardial energy metabolism， which might be one of the functionary mechanism of Yangxin2 Fang to treat CHF.
- chronic heart failure /
- Yangxin2 Fang /
- adenosine triphosphate (ATP) /
- mitochondrial membrane potential (MMP)

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