生地对高糖致人肾小球系膜细胞增殖、氧化应激及细胞外基质的影响
Rehmanniae Intervention of High-glucose Induced HRMC Proliferation and ECM Secretion
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摘要: 目的 观察生地含药血清对高糖刺激下人肾小球系膜细胞(HRMC)的作用。方法 制备生地含药血清,通过LC-MS检测技术,鉴别入血成分;MTT法检测HRMC增值抑制率;试剂盒检测细胞上清中SOD、MDA的含量变化;ELISA法检测HRMC中纤维连接蛋白(FN)、Ⅳ型胶原(ColⅣ);流式法检测细胞周期分布;免疫荧光检测HRMC中TGF-β1的表达。结果 生地含药血清中含有水苏糖、梓醇,含药血清中效应成分可抑制高糖诱导的HRMC增殖,减少细胞外基质(ECM)的增生,下调TGF-β1的mRNA表达。结论 生地可能通过调节TGF-β1的表达来抑制ECM的分泌从而改善糖尿病肾病的发生发展。Abstract: OBJECTIVE To investigate the effects of Rehmanniae-medicated serum on human glomerular mesangial cells (HRMC) stimulated with high concentration of glucose. METHODS Rehmanniae-medicated serum was prepared and analyzed for its serum components using LC-MS technology. MTT assay was used to detect the inhibitory rate of HRMC. Cell supernatant levels of SOD and MDA were measured by kits. ELISA assays were used to determine the contents of fibronectin and type IV collagen. Flow cytometry was used to analyze cell cycle distributions. Immunofluorescence was used to detect the expression of TGF-β1 in HRMC. RESULTS Rehmanniae-medicated serum contains catalpa alcohol and stachyose. These active components could inhibit high level glucose-induced HRMC proliferation, reduced the production of extracellular matrix, and downregulated the mRNA expression of TGF-β1. CONCLUSION Rehmanniae could inhibit ECM production by regulating TGF-β1 so as to prevent the development of diabetic nephropathy.
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Key words:
- Rehmanniae /
- serum containing drug /
- HRMC /
- ECM /
- TGF-β1
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