RP-HPLC法测定山茱萸环烯醚萜总苷中马钱苷和莫诺苷含量

李伟; 许惠琴; 陶玉菡; 农伟虎; 吴佳蕾

RP-HPLC法测定山茱萸环烯醚萜总苷中马钱苷和莫诺苷含量

1.
南京中医药大学药学院，江苏南京 210023
2.
江苏省中药药效与安全性评价重点实验室，江苏南京 210023

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出版历程
- 刊出日期: 2014-01-10

Simultaneous Determination of Loganin and Morroniside in Total Glucosides from Cornus officinalis by HPLC

1.
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China
2.
Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medical, Nanjing, 210023, China

摘要

摘要: 目的建立反相高效液相色谱法测定山茱萸环烯醚萜总苷中马钱苷和莫诺苷含量的方法，为完善山茱萸环烯醚萜总苷的质量评价提供依据。方法色谱柱：Lichrosphere C18柱（4.6 mm×150 mm，5 μm）；流动相：乙腈-水梯度洗脱（0～12 min，乙腈比例由10%增加到55%）；流速1.0 mL/min；柱温30 ℃；检测波长240 nm。结果马钱苷和莫诺苷分别在0.088～0.528 μg (r=0.999 8)和0.088～0.352 μg(r=0.999 5)之间范围内线性关系良好；平均回收率（n=6）分别为98.7%（RSD=1.3%）、98.6%（RSD=0.8%）。结论所建立的方法简便、准确、分离效果好、重复性好，可以用于控制山茱萸环烯醚萜总苷的质量。
- HPLC /
- 山茱萸 /
- 环烯醚萜总苷 /
- 马钱苷 /
- 莫诺苷
Abstract: OBJECTIVE To set up a method to simultaneously determine loganin and morroniside in total iridoid glycosides of cornus officinalis by HPLC， and to provide the basis for the quality evaluation. METHODS The analysis was performed on a Lichrosphere C₁₈ column and the mobile phase was composed of acetonitrile-water with a linear gradient elution. The flow rate was 1.0 mL/min， the temperature of column was 30 ℃， and the UV detection wave length was set at 240 nm. RESULTS Loganin and morroniside were linear in the ranges of 0.088~0.528 μg(r=0.999 8) and 0.088~0.352 μg(r=0.999 5)， respectively. The average recoveries were 98.7%（RSD=1.3%）and 98.6%（RSD=0.8%）. CONCLUSION The method of HPLC is accurate， easy to practice and reproducible， which can be used to control the quality of total iridoid glycosides of cornus officinalis.
- HPLC /
- cornus officinalis /
- total iridoid glycosides /
- loganin /
- morroniside

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参考文献(12)

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