四逆散、二仙汤及其合剂对慢性应激合并乳腺增生模型大鼠的影响

宋婷; 童钟; 李中平; 沈红艺

四逆散、二仙汤及其合剂对慢性应激合并乳腺增生模型大鼠的影响

1.
上海中医药大学基础医学院，上海 201203
2.
广东省工伤康复医院，广东广州 510970
3.
上海中医药大学健康营养研究室，上海 201203

计量
- 文章访问数: 699
- HTML全文浏览量: 1
- PDF下载量: 669
- 被引次数: 0
出版历程
- 刊出日期: 2014-01-10

Comparative Study of “Sini San” and “Erxian Decoction”on Rats with Chronic Psychological Stress and Mammary Hyperplasia Disease (MHD)

1.
College of Basic Medicine, Shanghai University of Chinese Medicine, Shanghai, 201203, China
2.
Guangdong Provincial Work Injury Rehabilitation Center, Guangzhou, 510970, China
3.
Department of Health Nutrition Shanghai University of Chinese Medicine Shanghai, 201203, China

摘要

摘要: 目的根据女性乳腺增生的发病与中医情志致病理论密切相关的特点，利用现代医学心身医学的理论与方法，探索复合因素即肝气郁滞兼冲任失调乳腺增生病证结合动物模型的造模方法，及中药治疗效果。方法随机将70只雌性未孕大鼠分为正常组10只，模型组15只，四增应组15只，二增应组15只，四加二增应组15只，采用慢性应激合并乳腺增生模型的造模方法，观察造模期间动物的行为学变化，造模后动物乳头组织高度并采用放免法检测血清等指标。结果造模方法对动物行为影响较大，各中药组治疗后动物体力和活动均有所改善，乳腺组织增生缓解。给予二仙汤、四逆散分煎及合煎治疗后，血清指标均有所恢复，组间无显著差异，合煎液较分煎液更降低E₂/P比值，缓解乳腺组织增生程度。结论四逆散、二仙汤均能缓解大鼠乳腺增生。慢性应激与乳腺增生复合造模，复制出病证结合动物模型产生了乳腺增生的效果，四逆散与二仙汤合煎与分煎在血清指标上无显著差异，调节雌/孕激素平衡作用合煎较分煎效果明显。
- 慢性应激 /
- 乳腺增生 /
- 疏肝解郁 /
- 调理冲任
Abstract: OBJECTIVE To explore effect of Chinese medicine on animal models with mammary hyperplasia disease induced by liver qi stagnation and Chong and Ren pulses disorder in the theory and method of psychosomatic medicine in modern medicine， according to the characteristic of setup of female breast hyperplasia closely related to the theory of pathogenic emotion leading to diseases in TCM. METHODS Combination of chronic stress and mammary hyperplasia was used to make models， during which the behavior changes， animal tissue papilla height after modeling were observed. And serum was detected in radioimmunoassay. RESULTS The modeling method made a big difference on animal behavior. Animal strengh and behavior were improved in each TCM group after treatment and breast tissue hyperplasia was relieved. After treatment with Erxian decoction， Sini powder and the combination， serum indexed were recovered and there were no significant difference among the groups. The ratio in group with the combination was lower E₂/P than that in group with seperated medicine. The hyperplasia was relieved in some degree. CONCLUSION Sini powder and Erxian decoction can improve breast hyperplasia in rats. Chronic stress and breast hyperplasia combined with animal models have induced the effect of breast hyperplasia. Sini powder， Erxian decoction and the combination make no significant differences in serum indicators; in terms of adjusting balance between estrogen and progesterone， the combination is better than the seperated.
- chronic stress /
- mammary hyperplasia /
- soothing liver and releasing depression /
- regulating Chong and Ren pulses

HTML全文

参考文献(9)

[1]	王雪琦，路长林，李丽云，等．大鼠抑郁模型的脑磁共振成像研究[J]．中华精神科杂志，1999， 32（1）：11-13．
[2]	Wang XQ， Lu CL， Li LY， et al. The nuclear magnetic resonance imaging of brain in the rats of depression model[J]. Chin J Physician， 1999， 32（1): 11-13.
[3]	郑兴东，王雪琦．抑郁症大鼠各脑区甘丙肽含量的变化及盐酸氟西汀对其的影响[J]．第二军医大学学报，2002， 23（6）：626-628．
[4]	Zheng XD， Wang XQ. Changes of galanin level in different brain areas and effects of fluoxetine hydrochloride on it in depressed rats[J]. J 2nd Milit Med Univ， 2002， 23(6): 626-628.
[5]	Kalynchuk LE， Pinel JP， Treit D， et al. Changes in emotional behavior produced by long-term amygdala kindling in rats[J]. Biol Psychiatry， 1997， 41(4): 438-451.
[6]	王洪彬，李峰，杨维益，等．元气精华调节情绪的实验研究[J]．河北中医，2002， 3（3）：234-236．
[7]	Wang HB， Li F，Yang WY， et al. Experimental study of the effect of Yuanqijinghua ricipe in regulating emotion[J]. Hebei J Tradit Chin Med， 2002， 3（3): 234-236.
[8]	赵长鹰，沈英森．金水六君煎抗应激作用的实验研究[J]．新中医，1997， 29（10）：35．
[9]	Zhao CY， Shen YS. Experimental study anti-stress of Jinshuiliujun Jian[J]. New J Tradit Chin Med， 1997， 29(10): 35.