黄芪、莪术配伍对人卵巢癌HO-8910原位移植瘤组织中MMP2和VEGF表达的影响

尹刚; 唐德才; 戴建国; 臧文华; 吴晓宇

黄芪、莪术配伍对人卵巢癌HO-8910原位移植瘤组织中MMP2和VEGF表达的影响

1.
南京中医药大学基础医学院，江苏南京 210023
2.
南京中医药大学附属医院，江苏南京 210029

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出版历程
- 刊出日期: 2014-01-10

Effect of Compatibility of Astragalus Plus Zedoary on the Expression of MMP2 and VEGF in Orthotopic Transplantation Tumor Tissue of HO-8910 Ovarian Carcinoma in Situ

1.
College of Basic Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China
2.
The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210023, China

摘要

摘要: 目的观察黄芪、莪术配伍对人卵巢癌HO-8910原位移植瘤抑瘤效果以及肿瘤组织中MMP2和VEGF表达的影响。方法选取已建立荧光蛋白转染的HO-8910卵巢癌原位癌动物模型（5组），分为模型组，阳性对照组（顺铂），黄芪组，莪术组，黄芪、莪术配伍组（按照2:1），动态测量各组肿瘤体积增长情况，观察抑瘤效果。用免疫组织化学法检测移植瘤组织中MMP2和VEGF蛋白的表达并计算表达率。结果黄芪、莪术配伍组以及顺铂组的移植瘤体积和瘤质量均小于模型组（P<0.05），黄芪、莪术配伍组移植瘤体积和瘤质量小于顺铂组、莪术组以及黄芪组（P<0.05），莪术组移植瘤体积和瘤质量与模型组相比较无统计学差异（P>0.05）。黄芪、莪术配伍组移植瘤体积和瘤质量小于顺铂组（P<0.05）；与模型组比较黄芪组，莪术组，黄芪、莪术配伍组和顺铂组移植瘤组织中MMP2和VEGF蛋白表达水平平均减弱（P<0.05）。结论黄芪、莪术配伍使用在实验周期内对人卵巢癌HO-8910抑瘤效果明显（P<0.05），其作用机制可能与下调MMP2和VEGF蛋白的表达有关。
- 黄芪 /
- 莪术 /
- 卵巢癌 /
- MMP2 /
- VEGF
Abstract: OBJECTIVE To observe the effect of Radix Astragali， Rhizoma Zedoariae and antitumor effect and the effect of MMP2 and VEGF expression in tumor tissues of human ovarian cancer HO-8910 orthotopic transplantation tumor. METHODS elect the HO-8910 ovarian carcinoma in situ animal models have been established fluorescent protein transfection(5 grope)，Including model group， positive control group (Shun Bo)， Astragalus group， Zedoary group， Astragalus，zedoary turmeric compatibility group (on the basis of 2∶1). Dynamic measurement of each tumor volume， tumor metastasis， tumor inhibitory effect. The protein expression of MMP2 and VEGF were deteced by immunohistochemical method. RESULTS Astragalus root， zedoary turmeric compatibility group and cisplatin group， tumor volume and tumor weight were lower than model group(P<0.05)， Astragalus， zedoary turmeric compatibility group tumor volume and tumor weight less than cisplatin group，Curcuma group and Astragalus group， Zedoary group of tumor volume and tumor weight compared with the model group（P>0.05）， Astragalus root， zedoary turmeric compatibility group tumor volume and tumor weight less than cisplatin group(P<0.05)， Compared with the model group the level of MMP2 and VEGF protein expression of average weakened Astragalus， zedoary turmeric compatibility group Astragalus group， Zedoary group and cisplatin group in transplanted tumor tissue(P<0.05). CONCLUSION In the experimental period，Astragalus root， zedoary turmeric compatibility which has obvious anti-tumor effect on human ovarian cancer HO-8910(P<0.05)，The action mechanism of the expression may be associated with the downregulation of MMP 2 and VEGF protein.
- Astragali /
- Zedoary /
- ovarian cancer /
- MMP2 /
- VEGF

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