乳香-没药药对中萜类成分的提取工艺优化研究

高茹梦; 张立雯; 缪晓冬; 宿树兰; 钱大玮; 欧阳臻; 段金廒

乳香-没药药对中萜类成分的提取工艺优化研究

1.
江苏大学药学院，江苏镇江 212013
2.
南京中医药大学江苏省中药资源产业化过程协同创新中心，中药资源产业化与方剂创新药物国家地方联合工程研究中心，江苏省方剂高技术研究重点实验室，江苏南京 210023

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出版历程
- 刊出日期: 2019-05-10

Study on Extraction Process of Terpenoids in the Mixture of Frankincense and Myrrh

1.
School of Pharmacy, Jiangsu University, Zhenjiang, 212013, China
2.
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China

摘要

摘要: 目的优化乳香-没药配伍药对中萜类成分的提取工艺。方法采用UPLC-TQ/MS联用技术，建立同时测定乳香、没药中10种萜类成分的定量方法；基于单因素试验考察提取方法、乙醇浓度、料液比、提取时间、提取次数的结果，设计正交实验优选乳香-没药药对中萜类成分的提取工艺参数。结果乳香-没药配伍药对中萜类成分的最佳提取工艺参数为：95%乙醇回流提取，料液比1∶12，提取2次，每次提取时间2 h。在该条件下，总萜类成分百分含量为10.44%。结论采用正交试验优化的提取工艺稳定可行，操作性强，可为乳香-没药配伍药对中萜类成分的提取制备提供参考，为其进一步开发利用提供科学依据与参考。
- 乳香-没药药对 /
- 提取工艺 /
- 萜类成分
Abstract: OBJECTIVE Optimization of extracting process of terpenoids in frankincense-myrrh. METHODS UPLC-TQ/MS technology was used to establish the determination method of 10 kinds of terpenoids in frankincense and myrrh; the orthogonal design was used to optimize the extraction process based on the results of single factor experiment on the extraction method， ethanol concentration， solid-liquid ratio， extraction time and extraction times. RESULTS The optimal extraction conditions were as follows: 12 times of 95% ethanol， refluxing and extracting for 2 h， totally extracting 2 times. The content of total terpenoids was 10.44%. CONCLUSION The extraction process optimized by orthogonal experiment is stable and feasible， which provide technical support for the further development and utilization.
- frankincense-myrrh /
- extraction process /
- terpenoids

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参考文献(14)

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