薤香方湿化氧疗对心肌梗死小鼠心肌损伤标志物和心肺组织病理形态的影响

林艳; 王琪; 宋少敏; 沈建平; 王新东

薤香方湿化氧疗对心肌梗死小鼠心肌损伤标志物和心肺组织病理形态的影响

林艳^1,2,
王琪^1,2,
宋少敏¹,
沈建平^1,2,
王新东^1,2,

1.
南京中医药大学附属中西医结合医院，江苏南京 210028
2.
江苏省中医药研究院心血管科，江苏南京 210028

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出版历程
- 收稿日期: 2017-11-11
- 刊出日期: 2018-03-10

Effect of Humidified Oxygen Therapy with Xiexiang Formula on Myocardial Injury Markers and Pathological Morphology of Cardiopulmonary Tissue of Mice with Myocardial Infarction

LINYan^1,2,
WANGQi^1,2,
SONGShao-min¹,
SHENJian-ping^1,2,
WANGXin-dong^{1,2
,}

1.
Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, 210028, China
2.
Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing, 210028, China

摘要

摘要: 观察薤香方湿化氧疗对心肌梗死小鼠心肌损伤标志物和心肺病理组织形态的影响，评估其对缺血心肌的保护作用和对肺组织的安全性。方法 40只C57BL/6雄性小鼠分为正常对照组、模型组、薤香方组（XXF组）及薤香方+挥发油组（XXFHF组）。ISO诱导建立心肌梗死模型。模型组给予50%氧气吸入，XXF组和XXFHF组分别给予薤香方和薤香方挥发油混合氧气湿化吸入，12 h/d，连续7 d。观察结束时，检测各组小鼠血清肌酸激酶（CK）、肌酸激酶同工酶MB（CK-MB）、乳酸脱氢酶（LDH）水平，超声心动图评价心功能及观察心肌和肺组织病理形态情况。结果与模型组比较，XXF和XXFHF组LDH、CK、CK-MB升高的幅度均较低（P＜0.05）；XXF组和XXFHF组LVEDD和LVESD值明显降低（P<0.05），EF和FS值明显增加（P<0.05）；心肌组织病理显示XXF和XXFHF组心肌坏死范围较ISO组轻，炎性细胞浸润和间质水肿亦较ISO组轻；4组肺组织均未见明显病理损伤性改变。结论薤香方湿化氧疗能够保护缺血状态下的心肌，对肺组织无病理性影响，辅助治疗AMI安全有效。
- 薤香方 /
- 氧疗 /
- 心肌梗死 /
- 心肌损伤标志物
Abstract: OBJECTIVE To observe the effect of humidified oxygen therapy with Xiexiang formula on myocardial injury markers and pathological morphology of cardiopulmonary tissue of mice with myocardial infarction， and to evaluate its protective effect on ischemic myocardium and its safety on lung tissue. METHODS 40 C57BL/6 male mice were divided into the normal control group (control group)， the model control group (ISO group)， the Xiexiang formula paste group (XXF group) and the Xiexiang formula paste + volatile oil group (XXFHF group). Myocardial infarction models were established through ISO induced method. ISO group was given 50% oxygen inhalation， XXF group and XXFHF group were given Xiexiang formula paste and Xiexiang formula paste + volatile oil mixed with humidified oxygen inhalation， respectively， 8 h/d for 7 d continuously. RESULTS The increase of LDH， CK and CK-MB in XXF group and XXFHF group were lower than that of the ISO group (P<0.05). LVEDD and LVESD in the XXF group and XXFHF group decreased obviously (P<0.05)， but EF and FS levels significantly increased (P<0.05). Myocardial histopathology in XXF and XXFHF groups showed small range of myocardial necrosis， the extent of necrosis was significantly lighter than that in the ISO group， and inflammatory cell infiltration and interstitial edema were also lighter than those in the ISO group. No obvious pathological changes were found in lung tissues of all four groups. CONCLUSION Humidified oxygen therapy with Xiexiang formula can protect myocardium in ischemic state， have no pathological effect on the lung tissue. As an adjunctive therapy， it is safe and effective in the treatment of AMI.
- Xiexang formula /
- oxygen therapy /
- myocardial infarction /
- myocardial injury markers

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