甘肃道地药材掌叶大黄药材的HPLC指纹图谱研究
Study on HPLC Fingerprint of Gansu Geoherbs Rheum palmatum
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摘要: 目的 建立甘肃礼县掌叶大黄的高效液相色谱指纹图谱,可为科学评价和有效控制其质量提供可靠的方法。方法 采用高效液相色谱法,色谱柱为Agela Venusil XBP-C18柱(4.6 mm×250 mm, 5 μm);流动相为乙腈-0.1%磷酸溶液,梯度洗脱;流速为1.0 mL/min;检测波长为254 nm;柱温为40 ℃。结果 掌叶大黄中所含主要成分的色谱峰均得到了有效的分离,在指纹图谱中共有峰有26个,并指认了5个色谱峰。结论 该方法对掌叶大黄质量控制以及实现规范化种植提供了参考依据。Abstract: OBJECTIVE To establish HPLC fingerprint of Rheum palmatum from Gansu and provide a reliable method for its scientific evaluation and quality control.METHODS The main components were separated on a Agela Venusil XBP-C18 (4.6 mm×250 mm, 5 μm) column and detected at 254 nm by gradient elution at the flow rate of 1.0 mL/min using acetonitrile-0.1% phosphoric acid as the mobile phase. Column temperature was at 40 ℃.RESULTS The main components of the samples harvested from 8 locations at Li County of Gansu were successfully and effectively separated and 26 peaks could be found from all chromatograms. 5 major peaks were identified based on the comparison with reference substances.CONCLUSION The method can be used for quality control and provide a valuable reference for Good Agriculture Practice of Rheum palmatum.
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Key words:
- Rheum palmatum /
- HPLC /
- fingerprint /
- quality control
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