竹叶总黄酮对UVB诱导HaCaT细胞氧化损伤的保护作用

高扬; 易若琨; 宋家乐

竹叶总黄酮对UVB诱导HaCaT细胞氧化损伤的保护作用

1.
江苏省苏北人民医院药剂科，江苏扬州 225001
2.
釜山国立大学食品科学与营养学科，韩国釜山 609-735
3.
桂林医学院公共卫生学院，广西桂林 541004

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出版历程
- 收稿日期: 2014-10-21
- 修回日期: 2014-12-30
- 刊出日期: 2015-03-10

Protective Effects of Total Flavonoids from Bamboo Leaves on UVB-irradiated Oxidative Damage in HaCaT Cells

GAOYang¹,
YIRuo-Kun²,
SONGJia-Le^{2,3
,}

1.
Department of Pharmacy, Northern Jiangsu People's Hospital, Yangzhou, 225001, China
2.
Department of Food Science and Nutrition, Pusan National University, Busan, 609-735, Korea
3.
Department of Nutrition and Food Hygiene, School of Public Health, Guilin Medical University, Guilin, 541004, China

摘要

摘要: 目的研究了竹叶总黄酮对紫外线B(UVB)诱发HaCaT角质细胞氧化损伤的保护效果和机制。方法 HaCaT细胞经竹叶总黄酮预处理24 h后，以辐射剂量为20 mJ/cm²的UVB照射细胞2 h后，MTT法测定细胞生存率。同时测定细胞内活性氧(ROS)水准，脂质过氧化程度，抗氧化酶(超氧化物歧化酶，SOD；过氧化氢酶，CAT和过氧化物歧化酶，GSH-Px)并利用RT-PCR法分析其mRNA转录水准。ELISA法分析TNF-α和IL-6分泌量。结果竹叶总黄酮可有效地抑制UVB导致的细胞氧化损伤并提高其细胞生存率。与对照组相比，竹叶总黄酮还能有效降低受损细胞内ROS水准，脂质过氧化程度，并提高SOD、CAT和GSH-Px等3种内源性抗氧化酶的活性及其它们的mRNA转录水平。此外，竹叶总黄酮还能抑制UVB所造成受损细胞分泌TNF-α和IL-6炎性细胞因子的能力。结论竹叶总黄酮可通过降低细胞内ROS水平，抑制细胞脂质过氧化，调节细胞内氧化酶的活性抑制UVB导致的HaCaT细胞氧化损伤。此外，竹叶总黄酮还可降低UVB所诱发的TNF-α和IL-6分泌。
- 总黄酮 /
- 竹叶 /
- HaCaT细胞 /
- UVB /
- 抗氧化
Abstract: OBJECTIVE To investigate the protective effects of total flavonoids from bamboo (Phyllostachys pubescens) leaves (BLE) on UVB- irradiated oxidative damage in HaCaT keratinocytes. METHODS The HaCaT cells were pretreated with BLE for 24 h and then exposed to UVB (20 mJ/cm²) for 2 h. Cell viability was determined by MTT assay. The levels of reactive oxygen species (ROS)， lipid peroxidation and antioxidant enzymes including catalase (CAT)， superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were measured. mRNA transcription levels of these antioxidant enzymes were determined by RT-PCR assay. In addition， the production of TNF-α and IL-6 were detected by ELISA assay. RESULTS BLE significantly reduced the UVB-induced HaCaT cell damage and improved the cell survival rates (P<0.05). Compared to the control group， BLE decreased the levels of ROS and lipid peroxidation， and increased the activities and mRNA transcription levels of endogenous antioxidant enzymes (SOD， CAT and GSH-Px) in UVB-irradiated HaCaT cells. In addition， BLE also inhibited the secretion of TNF-α and IL-6 in UVB-irradiated HaCaT cells. CONCLUSION BLE can exhibit cytoprotective activities against UVB-induced oxidative damage in HaCaT cells through the reduction of ROS， inhibition of lipid peroxidation and regulation of antioxidant enzymes activities. In addition， BLE can also decrease the levels of TNF-α and IL-6 in UVB-irradiated HaCaT cells.
- total flavonoids /
- Phyllostachys pubescens leaves /
- HaCaT cells /
- UVB /
- antioxidant

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