HPLC Specific Chromatograms of Margaritifera Concha<\i> from Different Area

OBJECTIVE To establish the specific chromatograms of Margaritifera concha by HPLC， so as to improve its quality control. METHODS The analysis was performed on an Agilent ZORBAX SB-C₁₈ column (4.6 mm×250 mm，5 μm) with a gradient mobile phase of acetonitrile-0.1% phosphoric acid at a flow rate of 0.5 mL/min. The detection wavelength was set at 254 nm and the column temperature was 30 ℃. Using phenylalanine as the reference solution， 10 batches of samples of Margaritifera concha were analyzed with the developed method，and similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (2012 Version) was used for the quality assessment. RESULTS Five common peaks were found through the similarity evaluation system. With phenylalanine as the reference， the relative retention time of the other four peaks were 0.703±0.006， 0.814±0.005， 0.881±0.001， 1.403±0.008， respectively. CONCLUSION The method has been validated by methodology， and can be used for the quality control of Margaritifera concha.