Identification of Pinellia Ternata<\i> and Its Counterfeits Based on HPLC Fingerprint Analysis

OBJECTIVE To develop a high performance liquid chromatography （HPLC） method for the fingerprint analysis of Pinellia ternata and its authenticity identification. METHODS HPLC analysis was performed on a SB-Aq column（4.6 mm×250 mm， 5 μm） with the mixture of 0.2% ammonium dihydrogen phosphate solution ( pH=3.0) (A) and methanol (B) as mobile phase in gradient mode. The column temperature was set at 30 ℃ and dual wavelength detection was used. RESULTS The HPLC fingerprint of Pinellia ternata，showing 10 characteristic peaks，was established from raw Pinellia ternata， and 5 were identified by standard comparison， and 8 common constituents were identified in fake Pinellia ternata samples. An overlay fingerprint were established with samples randomly selected： From 34 to 38 min，there were two characteristic peaks in the Pedate Rhizome Pinellia， and two appeared at 32 to 34 min in the Rhizoma Typhonii Flagelliformis prepared with ginger juice，while the peak height in the raw Typhonii flagelliformis Rhizoma was lower and the peak area was smaller. CONCLUSION The fingerprint of Pinellia ternata is significantly different from its confused herbs， which can be applied to the identification and quality control of Pinellia ternata.