Protection of Danggui Shaoyao San on the Down-Regulated Glucose Transporter in Mice Placental Microvillous Membrane by Controlled Ovarian Hyperstimulation

OBJECTIVE To assess the protection of Danggui Shaoyao San on the down-regulated glucose transporter in mouse placental microvillous membrane (MVM) by controlled ovarian hyperstimulation. METHODS IVF blastocysts were transferred to non-stimulated or superovulated pseudopregnant recipient mice. The non-stimulated pseudopregnant recipient mice were non-stimulated recipient mice group. The superovulated pseudopregnant recipient mice were superovulated recipient mice group. The superovulated pseudopregnant recipient mice which received Danggui Shaoyao San treatment were Danggui Shaoyao San treatment group. At E18.5 d， the fetal weight and placental weight were examined. The mRNA expressions of Igfbp1， Igf1and Glut1 in placenta were determined by qPCR. The IGFBP1 and IGF1 protein levels in placental homogenates were determined by ELISA. The GLUT1 protein levels in placental homogenates and MVM were detected by Western blotting. RESULTS At E18.5 d， the mean weights of fetal and placental (P<0.01)， the mRNA for Igf1、Glut1 in placenta (P<0.01)， and GLUT1 protein levels of placental homogenates and MVM (P<0.01) in superovulated recipient mice group were significantly lower compared with non-stimulated recipient mice group. The mRNA for Igfbp1 in placenta of superovulated recipient mice group was significantly higher compared with non-stimulated recipient mice group (P<0.01). Meanwhile， the mean weights of fetal and placenta (P<0.01) ， the mRNA for Igf1， Glut1 in placenta (P<0.05)， and GLUT1 protein levels of placental homogenates and MVM (P<0.05) in Danggui Shaoyao San treatment group were significantly higher compared with those in superovulated recipient mice group. The mRNA for Igfbp1 in placenta of Danggui Shaoyao San treatment group was significantly lower compared with that in superovulated recipient mice group (P<0.05). CONCLUSION Danggui Shaoyao San plays protective effect on the down-regulated glucose transporter in mouse placental MVM by controlled ovarian hyperstimulation. The protection of Danggui Shaoyao San is associated with down-regulated IGFBP1 protein expression and up-regulated IGF1 protein expression in placenta.