Effects of Effective Component of Kiwi Root on Inhibition of Proliferation and Migration of Gastric Cancer Cell BGC-823

OBJECTIVE To investigate the effects and mechanism of effective component of Kiwi root on inhibition of proliferation and migration of gastric cancer cell BGC-823. METHODS MTT to experiment the different concentration of Kiwi root polysaccharide with Kiwi root triterpene compounds， and to determine the best effective ratio， called Kiwi root effective component（KREC）; The scratch adhesion test and Transwell assay were performed to determine the migration and invasion capacity of the cells. Gene chip were used to analyze the gene which was associated with the inhibition of proliferation and migration of gastric cancer cell BGC-823; Quantitative PCR were used to examine the expression of miR-630. RESULTS When the concentration of Kiwi root polysaccharide is 0.5 g/L， the concentration ratio of the ursolic acid to the oleanolic acid is 8:1，there is a strong synergy between the components， which can be called KREC and can restrain the proliferation and migration of gastric cancer cell BGC-823.The results of Gene chip and Quantitative PCR showed that the inhibiting ability was related to the increase of miR-630. CONCLUSION KREC have inhibitory effect on proliferation and migration of gastric cancer cell BGC-823， which mechanism can be related to increased expression levels of miR-630.