Comparing Metabolites of Liguzinediol in Liver Microsomes among Different Species

OBJECTIVE To compare metabolites of liguzinediol in liver microsomes among different species， such as rats， dogs， monkeys and human. METHODS LC-MS/MS method was established to determine liguzinediol and its metabolites. The liver microsome incubation systems among different species were built to research the metabolites of liguzinediol. RESULTS Phase Ⅰ and phase Ⅱ metabolism of liguzinediol can be occurred in liver. The oxidation and glucuronidase acid binding products were all found in four kinds of liver microsomes. CONCLUSION The metabolites of liguzinediol in liver microsome from rats， dogs and monkeys were consistent with that from human. The results can be used to select laboratory animal for safety evaluation of liguzinediol in preclinical study.