WEIYu-jian, LOUBin, WANGXin-dong. Preparation of BaohuosideⅠ-loaded Mixed Micelles and Its Anti-tumor Activity[J]. Journal of Nanjing University of traditional Chinese Medicine, 2016, 32(1): 62-66.
Citation: WEIYu-jian, LOUBin, WANGXin-dong. Preparation of BaohuosideⅠ-loaded Mixed Micelles and Its Anti-tumor Activity[J]. Journal of Nanjing University of traditional Chinese Medicine, 2016, 32(1): 62-66.

Preparation of BaohuosideⅠ-loaded Mixed Micelles and Its Anti-tumor Activity

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  • OBJECTIVE Preparation of baohuosideⅠ-loaded mixed micelles and its anti-tumor activity. METHODS BaohuosideⅠ-loaded Solutol HS 15/Poloxamer 188 mixed micelles (BSPM) was prepared by thin-film hydration method and the preparation process of BSPM was optimized by orthogonal design. The formulation of BSPM was evaluated and the in vitro release behavior of BSPM was determined. Cell toxicity and uptake assays were employed to evaluate the anti-tumor activities. The targeting effect of BSPM was studied by fluorescence labeling. RESULTS The BSPM prepared showed spherical and regular shape, with an average particle size of 33.59 nm and its entrapment efficiency more than 80%. The 24 h-accumulated release ratio in vitro of BSPM was up to 82.56%. Cytotoxicity of BSPM on human breast cancer cell line MDA-MB-231 was significantly stronger than that of free baohuosideⅠ and the uptake rate was improved. CONCLUSION The BSPM which shows small particle size and high entrapment efficiency can enhance cell toxicity and uptake. It is an effective and promising formulation for anti-tumor drug.
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