Effects of NBUVB Combined with Tuibai Granule on CD4+/CD8+ Tlymphocyte Ratio and CD4+CD25+ Treg in the Peripheral Blood of Vitiligo Patients

OBJECTIVE To detect the expression levels of CD4+， CD8+ Tlymphocytes and CD4+ CD25+ Treg in the peripheral blood of patients with vitiligo after the treatment of NBUVB combined with Tuibai Granule， and then explore the relationship between vitiligo pathogenesis and the levels of CD4+， CD8+ Tlymphocytes as well as CD4+ CD25+ Treg. METHODS The expression levels of CD4+， CD8+ Tlymphocytes and CD4+ CD25+ Treg were detected by FlowCytometry among 120 cases of vitiligo patients and 30 cases of healthy control group. Then the 120 cases were further divided into three groups， with 40 cases in each group. Patients in the treatment group were treated with NBUVB combined with Tuibai Granule; Patients in the control groupⅠwere only given NBUVB， while those in the control groupⅡonly took Tuibai Granule. After 3 months treatment， the changes of the CD4+/CD8+ ratio and the expression levels of CD4+ CD25+ Treg were once again tested and the comparison was made among the four groups. RESULTS The CD4+ Tlymphocytes level in the peripheral blood decreased significantly， while the CD8+ Tlymphocytes level increased evidently. There were significant statistical differences when compared with the healthy control group(P<0.01); The CD4+/CD8+ ratio of the progressive stage was much lower than that of the stable stage(P<0.01); the CD4+/CD8+ ratio of the stable stage had no statistical difference when compared to that of the healthy control group(P>0.05). The frequency of CD4+， CD25+ Treg of vitiligo patients was significantly lower than that of healthy control group(P<0.01)， and the frequency of CD4+， CD25+ Treg of the progressive stage was also lower than that of the stable stage(P<0.01). After treatment with NBUVB combined with Tuibai Granule， the CD4+/CD8+ ratio and the frequency of CD4+， CD25+ Treg were significantly up-regulated compared with either the control groupⅠor the control groupⅡ(P<0.01，P<0.05). CONCLUSION These findings suggest that the abnormal level of CD4+/CD8+ ratio and the frequency of CD4+， CD25+ Treg might be involved in the vitiligo pathogenesis. NBUVB combined with Tuibai Granule can prevent vitiligo by regulating the CD4+/CD8+ ratio and the frequency of CD4+， CD25+ Treg.