Simultaneous Determination of three components in Compound Hu-huang Spray by UPLC

OBJECTIVE To develop a quantitative method simultaneously to determinate polydatin and baicalin in compound Hu-huang Spray by UPLC. METHODS The seperation was performed on a column Acquity UPLC BEH C18(100 mm×2.1 mm，1.7 μm). The mobile phase was composed of acetonitrile (A) and 0.05% H3PO4(B) with gradient elution. The flow rate was 0.4 mL /min with the wave length at 280 nm. The temperature was 35℃. RESULTS For polydatin， the calibration curve was linear (r=0.9992) in the range of 0.59~59.0 mg/L， the average recovery was 100.6% (RSD=1.56%). For baicalin， the calibration curve was linear (r=0.9999) in the range of 1.128～112.8 mg/L， the average recovery was 101.5% (RSD=1.68%). For imperatorin， the calibration curve was linear (r=0.999 9) in the range of 0.135～13.5 mg/L， the average recovery was 100.2% (RSD=3.03%). CONCLUSION The method was sensitive， accurate and can be used in the quality control of compound Hu-huang Spray.