Simultaneous Determination of Six Components in Compound Wuwei Buccal Tablets by HPLC

OBJECTIVE To establish a method to simultaneously determine puerarin， liquiritin， ammonium glycyrrhetate， schizandrol， deoxyschisandrin， and schizandrin B in compound wuwei tablet by HPLC. METHODS The elusion was performed on Kromasil C18(250 mm×4.6 mm， 5 μm） column. The mobile phase was composed of acetonitrile and 0.05% H3PO4 with gradient elution. The detection wave length was 250 nm， the flow rate was 1.0 mL/min and the column temperature was maintained at 30 ℃. RESULTS Puerarin， liquiritin， ammonium glycyrrhetate， schizandrol， deoxyschisandrin， schizandrin B are all linear in the range of 0.031 0~0.154 9，0.013 7~0.068 5，0.042 4~0.211 8，0.017 4~0.087 1，0.001 8~0.009 1，0.013 6~0.068 1 mg/mL respectively. Their average recoveries were 98.6%，100.4%，98.3%，98.3%，96.8%，99.4% and RSD of the above six components were 2.6%，2.8%，1.2%，2.3%，0.9%，2.5%. CONCLUSION The method is easy to perform and accurate and practical so that it can be used to determine the six components in the compound wuwei buccal tablets.