The Effects of Chuangliye decoction loading into modified collagen matrices on angiogenesis

OBJECTIVE To investigate the effects of Chuangling liquid and its loaded Collagen on angiogenesis.METHODS Different dosages of Chuangling liquid and its collagen loaded liquid were detected for through the chicken embryo allantois membrane model(CAM) by the optimal effects of angiogenesis. The vascular endothelial cell proliferation experiment (MTT)， the migration assay and the protein expression angiogenin1 (Ang1)， vascular endothelial growth factor (VEGF) and integrin(Integraav) with Western blot method were used for the detection of mechanism.RESULTS The CAM experiments showed that the angiogensis in 0.2 mL and 0.3 mL Chuangling liquid groups were significantly less than that of control group(P<0.05)， while there was no significant difference between 0.2 mL and 0.3 mL Chuangling liquid group. This antiangiogenic effects were reversed by the same dosage of Chuangling liquid loading into collagen with their effects reaching to the effects of control group. The 0.2 mL dosage of Chuangling liquid loading into collagen and/or loading into the collagen were chosen for the further experiment. The HUVEC proliferation rate of control group increased 86%， as compared with the group of Chuangling liquidwhich decreased. After loading into collagen， the proliferation rate was increased significantly higher than the results of control group with about 24%(P<0.01). The cell migration movement of 12hours， 24hours in the group of Chuangling liquidwere significantly lower than those in other group. When loading into collagen， cell migration rate began to accelerate and the migration rate significantly faster than that of control group on 24hours(P<0.01); Mechanism detection showed that the protein expressions of Chuangling liquidwith Integraаv was significantly lower than the results of control group， and the higher of Ang1 expression. After loading into collagen， the protein expressions of Integraаv， VEGF and Ang1 were increased significantly.CONCLUSION The study showed that negative effects on vascular endothelial cell migration and proliferation and thus inhibiting angiogenesis. Chuangling liquid loaded into Collagen may reduce the inhibition to angiogensis and be beneficial for blood vessels formation， in order to improve wound healing.