Effect of Anti-heart-failure Granule on RAAS and TGF-β1 in of Rats with Diastolic Heart Failure

OBJECTIVE To observe the effect of anti-heart-failure granule on plasma renin activity(PRA)， angiotensinⅡ(AT-Ⅱ)， aldosterone (ALD) and transforming growth factorβ1(TGF-β1) of rats with diastolic heart failure. METHODS With Abdominal aorta constriction to establish model of diastolic heart failure rat， rats were randomly divided into six groups， namely model group， benazepril group， large， medium and small dose of anti-heart-failure granule groups as well as sham-operation group. PRA， AT-Ⅱand ALD of the rats in different groups were tested. The expression of TGF-β1 in cardiomyocytes was carefully detected respectively by immunofluorescence staining techniques and Western-Blot analysis.RESULTS Compared with the model group， AT-Ⅱof benazepril group and anti-heart-failure Granule large dose group decreased(P<0.05); ALD of benazepril goup and anti-heart-faiure Granule large， medium groups decreased(P<0.05). After detection by immunofluorescence staining techniques， it indicated that TGF-β1 of Benazepril group and Anti-heart-failure Granules groups were lower than model group(P<0.05)， while after Western-Blot analysis， it showed that the expression of TGF-β1 of Benazepril group and Anti-heart-failure Granules large dose group was suppressed compared with the model group (P<0.05).CONCLUSION Anti-heart-failure granule may reduce AT-Ⅱand ALD of rats with diastolic heart failure and also may suppress the expression of TGF-β1 in cardiomyocytes.