Effect of Puerarin on Glucose and Lipid Metabolism in Gestational Diabetic Mellitus Rats

OBJECTIVE To observe the influence of puerarin on glucose and lipid metabolism in gestational diabetic mellitus(GDM) rats. METHODS High-fat feeding pregnant wistar rats were given streptozocin （STZ） 25 mg/kg intraperitoneally on the first day of pregnant. After injection for 72 hours， rats with FBG between 6.67-16.67 mmol/L were gestational diabetic models， which were randomly divided into two groups: GDM model group(MG， using 0.9% NaCl)， puerain treated group(TG， using puerain 0.25 g/(kg·d)， 6 rats in each group. And we selected 6 pregnant rats as normal gestation group(NG， using 0.9% NaCl). The rats were given drugs by intragastric administration until the offspring were born. The levels of FBG， insulin， serum lipid， TNF-α， IL-6， leptin and adiponection were observed at the age of 25 weeks. Leptin receptor insulin receptor， TNF-α， IL-6， adiponection and leptin mRNA expression in fat and liver tissues were detected by qPCR. RESULTS Compared with GDM model group， the levels of FBG， insulin， TG， TC， LDL-C， TNF-α， IL-6 and leptin of puerarin treated group were significantly decreased(P<0.05， P<0.01). The levels of HDL-C and adiponection of puerarin treated group were significantly increased(P<0.05， P<0.01). The mRNA expression of IR in TG was higher than MG， and the mRNA expression of TNF-α and leptin in TG were decreased significantly （P<0.05）. CONCLUSION Puerarin can improve glucose and lipid metabolism in gestational diabetic rats， the mechanism may be related to the regulation of TNF-α， IL-6， leptin and adiponectin levels.