Renal Protective Effect of Absorbed Bioactive Compounds of Danggui Buxue Decoction on GK Rats

OBJECTIVE To observe renal protective effect and possible mechanism of absorbed bioactive compounds (ABCs) of Danggui Buxue Decoction on GK rats. METHODS The qualitative and quantitative analysis of ABCs of Danggui Buxue Decoction were performed by UPLC-MS/MS and the composition of ABCs were determined. Then the ABCs concentration of 1 g mother decoction was quantified. GK rats were randomly divided into model group， decoction group and ABCs group (n=10). Treatment groups were administered Danggui Buxue Decoction (4 g/kg) or ABCs (equal to the content of decoction， caffeic acid 5.36 mg/kg， formononetin 2.68 mg/kg， calycosin 2.56 mg/kg， ferulic acid 1.36 mg/kg， astragaloside 0.45 mg/kg，butyphthalide 0.16 mg/kg， ligustilide 0.16 mg/kg)， once a day for 28 d. 10 Wistar rats were chosen for control group and treated with vehicle (saline). Observation index: urinary total protein and urinary albumin， oxidative stress in kidney and the pathological observation of kidney. RESULTS Danggui Buxue Decoction and ABCs significantly decreased urinary total protein， urinary albumin of GK rats and the level of MDA of kidney(P<0.05)， significantly increased the activity of SOD of kidney(P<0.05) and displayed a protective effect on kidney pathomorphology. CONCLUSION ABCs can represent Danggui Buxue Decoction play a renal protective effect on GK rats， the mechanism involving oxidative stress. However， except for ABCs， the trace elements， amino acids， polysaccharides and other ingredients in mother decoction also play significant roles.