The influence of Astragalus Membranaceus on Calcium and Phosphorus Metabolism and FGF23-Klotho Axis in Rats with Chronic Kidney Disease

OBJECTIVE To observe the expression change of FGF23-Klotho axis on calcium and phosphorus metabolism in rats with chronic kidney disease，and the influence of Astragalus membranaceus on them. METHODS 30 SD rats were randomly divided into three groups（control group，model group and AM group），after adaptive feeding for 1 week， 250 mg/kg adenine suspension by gastrogavage and 1.2% phosphorus content by dietary were executed to molding for 4 weeks. Then， adenine gavage was given once in 2d in the following 2 weeks; at the same time， the rats in AM group were given AM decoction 12.5 g/(kg·d) gavage and control group & model group were given normal saline equivalent. Before dosing，selected 3 rats randomly in each group to detect the level of blood urea nitrogen(BUN)，creatinine(Scr)，calcium(Ca)，phosphorus(P) via heart puncture blood.Meanwhile，the parathyroid hormone(PTH)，FGF23，Klotho and 25(OH)D₃ were detected by ELISA.After 6 weeks，the histological changes in renal were observed by HE staining，the level of BUN，Scr，Ca，P in serum were detected via abdominal aortic blood.Meanwhile，PTH，FGF23，Klotho and 25(OH)D₃ were detected by ELISA. RESULTS Compared with the control group，the qualities of kidney and theBUN、Scr、Ca、P levels significantly increased in model group （P<0.01，P<0.05），while the PTH level not markedly elevated. Compared with the model group， the qualities of kidney and theBUN、Scr、Ca、P levels were decreased in AM group，while the PTH level not markedly elevated，and the BUN、Ca、P levels were statistical difference（P<0.01，P<0.05）.HE staining showed that the number of normal glomerular improved， compared with the model group， the expansion degree of renal tubular decreased， the number of inflammatory cells decreased， and the crystallization of adenine decreased in the BHD group.Compared with the control group，the FGF23 level increased in model group，while the Klotho and 25(OH)D₃ decreased.Compared with the model group，the FGF23 levelnot markedly elevated（P<0.05），and the Klotho not markedly declined（P<0.05）. CONCLUSION Astragalus membranaceus could alleviate the disorder of calcium and phosphorus metabolism in CKD rats，inhibit the high expression of FGF23，and up-regulate the expression of Klotho，thus improving histology of kidney， kidney function，and the balance metabolism of Ca and P in CKD rats.