Effect of Huangqi Baoxin Decoction on Ventricular Remodeling in Rats with Dilated Cardiomyopathy Induced by Adriamycin

OBJECTIVE To preliminary discuss the influence and mechanism of Huangqi Baoxin Decoction on ventricular remodeling in rats with dilated cardiomyopathy (DCM) induced by adriamycin. METHODS 90 SD rats were given intraperitoneal injection of adriamycin to establish the DCM model， with 55 rats model being succeed. Then they were randomly divided into model group (the same volume of physiological saline solution)， low-dose group with Huangqibaoxin Decoction (5.67 g/kg)， middle-dose group with Huangqibaoxin Decoction (11.34 g/kg)， high-dose group with Huangqibaoxin Decoction (22.68 g/kg)， and captopril group (6.75 mg/kg of captopril)， with 11 rats in each group. The rats were given orally administrated drugs once a day， with 10 rats in the normal control group being treated with the same volume of physiological saline solution. 4 weeks later， rats in each group went through the echocardiography to access the construction and function of their left ventricular. Crotid artery intubation was applied to monitor their hemodynamic indexes， with changes of myocardial tissue morphology being observed. Besides， NT-proBNP， ST2， AngⅡ， ALD and Renin were tested through ELISA， together with the activity of SOD， MDA， and LDH being tested via colorimetric method. RESULTS Compared with the normal group， the LVEDD and LVESD in rats of the model group were increased， with EF and FS being decreased (P<0.01). The LVDP，±dp/dtmax went down， while the LVEDP went up(P<0.01).The heart weight index increased(P<0.01)， with the myocardial cells being arranged in disorder and massive hyperplasia of fibrous tissue. The NT-proBNP， ST2， Renin， AngⅡ and ALD level increased(P<0.01). The level of SOD decreased， while the content of LDH and MDA increased (P<0.01). Compared to the model group， LVEDD and LVESD levels in the low， middle and high dose group of Huangqi Baoxin Decoction were reduced to varying degrees， with increased EF and FS (P<0.05~0.01). They experienced an increased LVDP，±dp/dtmax and decreased LVEDP(P<0.05~0.01)， decreased HWI and LVMI(P<0.05~0.01). And the myocardial cells were neatly arranged， the hyperplasia of fibrous tissue were relived. Besides， the levels of NT-proBNP， ST2， Renin， AngⅡ and ALD went down on diverse degree(P<0.05~0.01)， with decreased LDH and MDA and increased SOD(P<0.05~0.01). There was a dose-effect relationship among the groups of different doses towards the improvement on the above indexes. CONCLUSION Huangqi Baoxin Decoction can improve the cardiac hypertrophy and fibrosis in rats with dilated cardiomyopathy. It also improves the cardiac function and inhibits ventricular remodeling by regulating the oxidation factors and inhibiting the activation of RAAS neuroendocrine factors.