Study on Simultaneous Determination of Saikosaponin a， Aloe-emodin， Rhein， Emodin， Chrysophanol， Emodin and Hesperidin in Xiaozheng Pills with HPLC

OBJECTIVE To apply HPLC for simultaneous determination of Saikosaponin a， aloe-emodin， rhein， emodin， chrysophanol， emodin and hesperidin in Xiaozheng pills. METHODS An HPLC method was established. Waters Xbridge C₁₈ column（4.6 mm×250 mm，5  μm）was used as analytical column with a mobile phase consisting of acetonitrile (A)-0.3% phosphoric acid (B). The flow rate was 0.8 mL/min， detection wavelengths λ₁ = 210 nm and λ₂ = 300 nm and the column temperature 40 ℃. RESULTS The seven ingredients all reached baseline separation and were determined. CONCLUSION HPLC method is accurate， reliable and repeatable， which can provide a more comprehensive basis for quality control of Xiaozheng pills.