Impact of Jianpi Qinghua Fang on Renal Function， Proteinuria and Kidney Col-IV Expression in Rats with Renal Fibrosis

OBJECTIVE To investigate the impact of Jianpi Qinghua Fang on renal function， proteinuria and kidney Col-IV expression in focal segmental glomerulosclerosis rats induced by nephrectomy combined with adriamycin. METHODS The model of focal segmental glomerulosclerosis was prepared by the unilateral nephrectomy and the two injection of adriamycin in caudal vein of rat at a dose of 3 mg/kg. The gastric infusion with Jianpi Qinghua Fang， the disassembled prescription of Jianpi Qinghua Fang and Niaoduqing Capsule were administered separately for 8 weeks. The renal function and 24 h proteinuria were determined. The expression of Kidney Col-IV was determined by Real-Time Q-PCR. RESULTS Compared with control group and sham operation group， levels of SCr， BUN， 24 h proteinuria and kidney Col-IV expression in animals of model group were significantly higher (P<0.01). Compared with model group， in the group with Jianpi Qinghua Fang， levels of SCr， BUN， 24 h proteinuria and kidney Col-IV expression decreased significantly (P<0.01). Compared with model group， in the group with Qingre Huashi prescription， level of BUN decreased (P<0.05)， while levels of SCr and kidney Col-IV expression decreased significantly (P<0.01). Compared with model group， in the group with Niaoduqing Capsule， levels of SCr and 24 h proteinuria decreased (P<0.05) while levels of BUN and kidney Col-IV expression decreased significantly (P<0.01). Compared with model group， in the group with Jianpi Yiqi prescription， levels of SCr， BUN， 24 h proteinuria and kidney Col-IV expression unchanged (P>0.05). CONCLUSION Jianpi Qinghua Decoction could inhibit the accumulation of extracellular matrix and the proliferation of glomerular mesangium by lowering the expression of Col-IV in kidney tissue.Therefore，the renal fibrosis was relieved by Jianpi Qinghua Decoction，and the residual renal function was protected.The disassembled prescription Qingre Huashi prescription has the close curative effect in improving the renal function and renal fibrosis，prompting herbs of clearing heat and removing dampness is the key of Jianpi Qinghua Decoction against renal fibrosis.