Volume 29 Issue 2
Mar.  2013
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ZHOUNong, PANGJie, HUWen-shuai, WANGYong-xiang. Study on HPLC Fingerprint of Gansu Geoherbs Rheum palmatum[J]. Journal of Nanjing University of traditional Chinese Medicine, 2013, 29(2): 187-191.
Citation: ZHOUNong, PANGJie, HUWen-shuai, WANGYong-xiang. Study on HPLC Fingerprint of Gansu Geoherbs Rheum palmatum[J]. Journal of Nanjing University of traditional Chinese Medicine, 2013, 29(2): 187-191.
shu

Study on HPLC Fingerprint of Gansu Geoherbs Rheum palmatum

  • 1.

    College of Life Science and Engineering, Chongqing Three Gorges University, Chongqing, 404000, China

  • 2.

    College of Pharmacy , Dali University, Dali, 671000, China

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  • Received Date: November 24, 2012
  • Revised Date: January 02, 2013
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    • Abstract
  • OBJECTIVE To establish HPLC fingerprint of Rheum palmatum from Gansu and provide a reliable method for its scientific evaluation and quality control.METHODS The main components were separated on a Agela Venusil XBP-C18 (4.6 mm×250 mm， 5 μm) column and detected at 254 nm by gradient elution at the flow rate of 1.0 mL/min using acetonitrile-0.1% phosphoric acid as the mobile phase. Column temperature was at 40 ℃.RESULTS The main components of the samples harvested from 8 locations at Li County of Gansu were successfully and effectively separated and 26 peaks could be found from all chromatograms. 5 major peaks were identified based on the comparison with reference substances.CONCLUSION The method can be used for quality control and provide a valuable reference for Good Agriculture Practice of Rheum palmatum.
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    • References (12)
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