Danggui Yinzi's Effect on the Related Cytokines Abnormally Secreted by T cells of Chronic Urticaria belonging to Qi and Blood Deficiency Syndrome

OBJECTIVE To study the therapeutic mechanism of Danggui Yinzi on chronic urticaria belonging to qi and blood deficiency syndrome.METHODS KM mice with equal number of male and female were given reserpine and acetophenone hydrazine to duplicate the model of qi and blood deficiency syndrome， and joint type IV allergic reaction imitated urticaria model. The successful models were selected and randomly divided into model group， loratadine group， high dose of modified Danggui Yinzi group， middle dose of modified Danggui yinZi group and low dose of modified Danggui Yinzi group， 8 animals for each， the rest 8 were taken only as control group. At the end of experiment， blood was taken from orbit of animals of each group， lens detected peripheral eosinophil (EOS) change after serum separation; serum IgE content was tested by ELISA; the expression quantity of hemal wall organization IL-4， IFN-γ was tested by immunohistochemical detection through cutting vascular wall organization.RESULTS Compared with the model group， Danggui Yinzi can significantly reduce the animal peripheral eosinophil in every dose group (P<0.01); It can reduce serum IgE content (P<0.01); It can reduce hemal wall organization IL-4(P<0.01)， It also can elevated IFN-V expression (P<0.01).CONCLUSION Danggui Yinzi can inhibit urticaria through intervening abnormal secretion of related cytokines in T cells.