The Study of Euryale Seed Extracts Protecting SH-SY5Y Neurocytes Damage and Their Invitro Antioxidant Activities

OBJECTIVE To investigate the protective effects of euryale seed 80% ethanol， ethyl acetate and n-butyl alcohol extracts on the H₂O₂-induced damaged SH-SY5Y neurocytes and their invitro antioxidant activities.METHODS The H₂O₂-induced damaged SH-SY5Y neurocyte models were cultured outside of the body. Cell Counting Kit-8 (CCK-8) was used to determine the effects of the above euryale seed extracts with different concentrations on the activities of the H₂O₂-induced damaged SH-SY5Y neurocytes， and then their inhibition rates were calculated. The in-vitro antioxidant experimental method was applied to detecting the capabilities of the three extracts in removing DPPH， ABTS radicals and deoxidating three price iron ions.RESULTS Within the crude drug concentration range of 10~30mg/mL， euryale seed extracts in 80% ethanol， ethyl acetate and n-butyl alcohol showed obvious functions of inhibiting the oxidant damage action of H₂O₂ on the SH-SY5Y neurocytes (P<0.05， P<0.01). In addition， the above euryale seed extracts could markedly clear away DPPH， ABTS radicals and deoxidate three price iron ions， and among which， the n-butyl alcohol extract had the most powerful action.CONCLUSION Having an excellent antioxidant activity， euryale seed can protect the SH-SY5Y neurocytes from the damage of H₂O₂. Its active extracting solvents include ethanol， ethyl acetate and n-butyl alcohol. After a comprehensive comparison， it shows that the n-butyl alcohol extract has the most powerful function.