Simultaneous Determination of 7 Active Components in Qianyang Yuyin Prescription by UPLC

OBJECTIVE An UPLC method in vitro was established for simultaneous determination of seven active components in Qianyang Yuyin prescription， including gallic acid， protocatechuic acid， loganin， stilbene glucoside， cyasterone， cinnamic acid and harpagoside. METHODS The analysis was performed on an Waters H-Class UPLC C18 column (2.1 mm×100 mm， 1.7 μm) eluted with a gradient mobile phase of acetonitrile-0.1% formic acid solution system at a flow rate of 0.3 mL/min. The detective wavelength was set at 250nm， the column temperature was maintained at 30℃ and the injection volume was 2 μL. The 7 main chemical compounds in Qianyang Yuyin prescription were determined by UPLC. RESULTS The linear relationship of the 7 chemical constituents in Qianyang Yuyin Prescription were good in their respective concentration ranges(r>0.997 5)， The average recoveries were 95.8%~103.75%， the RSD of average recovery were 0.1%~1.0%， the RSD of precision were 0.10%~0.56%， the RSD of stability were 1.25%~9.04% and the RSD of reproducibility were 1.01%~3.24%. CONCLUSION The new established method in Qianyang Yuyin prescription was simple， accurate and reliable， which will supply instruction and reference to the internal Pharmacokinetics of Qianyang Yuyin prescription in the late works of this issue.