Metabolomic Approach to Evaluating Fetal Toxicity of Pinellia Rhizoma and Pinelliae Rhizoma Praeparatum cum Zingibere et Alumina Based on BeWo in vitro Placenta Model

OBJECTIVE To evaluate the potential developmental toxicity of Pinellia Rhizoma(BX) and Pinelliae Rhizoma Praeparatum cum Zingibere et Alumina(JBX) by analyzing the changes of intracellular metabolites after the intervention of BX and JBX by gas chromatography-mass spectrometry (GC-MS). METHODS The BeWo cell line derived from placenta tissue was used to construct the model of the in vitro placenta， and the changes of the metabolites and their relative contents in the cells were detected by GC-MS after the intervention of BX and JBX. RESULTS The nine metabolites of glycine， alanine， proline， glucose， galactose， stearic acid， inositol and so on were identified. CONCLUSION GC-MS technology is feasible for evaluation of developmental toxicity of BX and JBX.