Regulatory Effect of JianpiBushen Recipe on the Secretion Function and Expressions of NF-κB and MMP-9 in Placental Tissues of Pregnant Rats with Subclinical Hypothyroidism

OBJECTIVE To study the effects of JianpiBushen recipe(JBR) on the secretion function and Nuclear Kappa B(NF-κB) and matrix metalloproteinase-9（MMP-9）in placental tissues of pregnant rats with subclinical hypothyroidism(SCH) and investigate the protective mechanism of JBR. METHODS Ninety female adult Wistar rats were randomly divided into 2 groups: normal control group(false thyroid surgery was carried out， n=10) and model control group(total thyroidectomy was carried out， n=80).The animal model of SCH was established by using＂total removal of the rats thyroid 30 days， levothyroxine was given to the rats by subcutaneous injection at 9.5μg/(kg·d)＂， All rats in the model with overt hypothyroidism were selected 10 as low dose Chinese medicine before pregnant group(levothyroxine was given to the rats by subcutaneous injection at 9.5μg/kg·d and low dose Chinese medicine， Q)， others were injected levothyroxine subcutaneously at 9.5μg/(kg·d) for 9 days， serum TSH and TT4 were detected by radioimmunoassay on the tenth day and screening of SCH rats， the ratio of male to female=2∶1 was used as a cage. Through the observation of vaginal suppository and vaginal smear to find sperm to determine whether the mating and determine D0 day. Then randomly divided into 4 groups: ①The control group is SCH group(C) ②Low dose Chinese medicine group(L) ③High dose Chinese medicine group(H) ④Western medicine group(X). Serum chorionic gonadotropin (HCG)， estradiol (E₂) and progesterone (P) of the pregnant rats with SCH on the 10*day after conception (D10) in each group were detected by radioimmunoassay； The expressions of NF-κB and MMP-9 in placental tissues of pregnant rats with SCH on D10 in each group were detected by immunohistochemistry and Westernblottingassay. RESULTS Compared with the C group， on the tenth day， the serum levels of P and HCG in each treatment were all significantly higher (P<0.01)， the serum levels of E₂ were significantly higher in the X group(P<0.05)， The results of immunohistochemistry and Western Blot showed that the expressions of NF-κB and MMP-9 in placental tissues in each treatment group were all significantly higher (P<0.05). CONCLUSION JBR can promote the secretion of placenta and increase the expressions of NF-κB and MMP-9 in the placenta of pregnant rats with SCH.