Protective Effects and Mechanism of Polysaccharide from Platycodon Grandiflorum on Damage of PC12 Cells Induced by H₂O₂

OBJECTIVE To determine the protective effects of Platycodon grandiflorum polysaccharide (PGP) on PC12 cells injured by H₂O₂ and the underlying mechanisms. METHODS The cell viability was analyzed by MTT assay， the model was established by treating PC12 cells with H₂O₂. The levels of LDH， MDA， SOD and GSH-Px were determined by Kits. ROS production was determined by DCFH-DA fluorescence. mRNA and proteins of NOX2， p22* and Rac were analyzed by qPCR and Western Blot methods. RESULTS Compared with the model， treatment with PGP significantly decrease of LDH， MDA and intracellular ROS production， as well as increase activity of SOD and GSH-Px. Furthermore， PGP could down-regulate mRNA and proteins of NOX2， p22* and Rac. CONCLUSION PGP exhibited protective effect on PC12 cells injury induced by H₂O₂， it may via the suppression of NOX2 activation.