Study on Experimental Anti-adhesion Effect of Huoxue Tongfu Fang and the Mechanism

OBJECTIVE 60 male SD rats were randomly divided into six groups: normal control group， model group， low， medium and high dose of Huoxue Tongfu Fang groups and dexamethasone sodium phosphate positive control group. All groups were prepared for adhesion models except normal control group; control group and model group were injected with saline; dexamethasone group received intraperitoneal injection of dexamethasone sodium phosphate (10 mg/kg); low， medium and high doses of Huoxue Tongfu Fang groups orally administered Huoxue Tongfu Fang (0.65， 1.3， 2.6 g/100 g) a week for all. On the 8th day， blood in each group was taken out and IL-6， IL-1β and TNF-α in serum were determined in ELISA method; adhesion tissue was taken and TGF-β1 and CTGF levels were detected with ELISA kit after homogenate; adhesion degree was recorded. The expression of FN mRNA in adhesion tissue was tested in RT-PCR; p-Smad 3 protein level was detected in Western blot. RESULTS IL-1β and TNF-α in model group increased; so did the expression of FN mRNA， TGF-β1 and CTGF levels. Compared with model group， the degree of adhesion in all doses of Huoxue Tongfu Fang significantly reduced; IL-1β and TNF-α in serum and TGF-β1 and CTGF levels significantly declined (P<0.05~0.01); the expression of FN mRNA and p-Smad 3 decreased (P<0.05~0.01). CONCLUSION Huoxue Tongfu Fang can reduce the degree of adhesion， making a preventive and therapeutic effect on adhesion.