Establishment of Adriamycin Glomerule Sclerosis Models Assisted by High-lipid Fodder

OBJECTIVE To establish animal models for focal segmental glomerulosclerosis.METHOD Nephrectomy of the right kidney of SD rats was performed,followed by caudal vein injection of adriamycin twice(3mg/kg each time) and feeding of high lipid fodder.RESULT This model is manifested by edema, large amount of albuminuria,hypoproteinemia and hyperlipemia.Pathological examinations,computer image analysis and immunohistochemical staining suggested that the glomerule was manifested by the increase of the mesenterium base,focal segmental glomerulosclerosis,and an increase in the expression of extracellular matrix FN and Col IV.CONCLUSION This model provides the necessary conditions for the study of the pathogenesis of chronic diseases of the glomerule.