Cyto-membrane Immobolized Chromatography:Hypothesis,Method and Application in Traditional Chinese Medicine

A wide variety of molecules,including bormones,drugs,neurotransmitters,growth factors,antigens,viruses,plasma lipoproteins and glycoproteins,elicit their biological effects by virtue of their binding to cellular receptor.Many of these agents,however,interact with receptor or other target on external surface of the cell imbedded in cellular plasma membrane.Although in most circumstance,we still do not know correctly which substance are responsible for the effects of Traditional Chinese Medicine,and through which mechanism it works.But based the clinical response and pharmacological experimental phenomena,we believe that most Traditional Chinese Medicine's effect come from their compnents which interact with target of the organism.It is generally that noncovalent binding of drug to the target on the cell membrane may appear to be essentially reversible.Receptor and other target extend through the cell membrane.The part of the target on the exterior of the cell surface capture the signal components of TCM to be communicated to the cell in the outside environment.So our working hypothesis has been put forward as:The cell membrane of organism could be used as a material with which effective chemicals in Traditional Chinese Medicine could bind to the target that embedded on it.That is basis of cyto-membrane immobilized chromatography and it's application in Traditional Chinese Medicine.The cyto-membrane lmmoboliged chromatography is composed of the biologic membrane immobilized part and the chromatography part,The apavts have beon separatedin our wark,both parts have their optimal situation.This may be the creational in our work.The applications of red blood cell membrane immobilized chromatography in Angelica archangelica,platelet membrane immobilized chromatography in Salviae miltiorrhizae radix and Mailuoning Injection,a formula herbal injection for blood stasis,have been analyzed.The results shown that cyto-membrane immobilized chromatography are worked,although it is a pilot job.