Abstract: OBJECTIVE To study the regulatory effect of Hedan Tiaozhi Recipe on abnormal plasma lipid metabolism in hyperlipidemia mice. METHODS By using UPLC-LTQ/Orbitrap XL， the effect of drug intervention on lipid metabolizer group was investigated， potential biomarkers were searched， and the action way of Hedan Tiaozhi Recipe in regulating lipid metabolism was explored. RESULTS Mass spectrometry of plasma lipids of normal group， model group and Hedan Tiaozhi Recipe group were all significantly separated. 11 biomarkers were identified in the plasma， mainly including phosphatidylcholine， lysophosphatidylcholine and triglycerideincluding phosphatidylcholine. The intervention of Hedan Tiaozhi Recipe significantly improved some abnormal lipid metabolism. CONCLUSION Hedan Tiaozhi Recipe can effectively alleviate the metabolism disorder of phosphatidylcholine， lysophosphatidylcholine and triglyceride induced by high-fat diet， which indicated that the mechanism of Hedan capsule regulating blood lipid may be related to the regulation of these metabolic pathways.