HPLC指纹图谱及多成分定量的独活饮片质量评价研究
Quality Evaluation of Angelica Pubescens Radix Pieces by HPLC Fingerprint and Multi-Component Quantitative Analysis
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摘要: 目的 建立独活饮片HPLC指纹图谱，并同时测定6种主要成分的含量，为其饮片炮制及质量控制提供依据。方法 HPLC-PDA法建立15批独活饮片的指纹图谱，结合聚类分析和偏最小二乘法判别分析（PLS-DA），对15批独活饮片进行质量分析，测定二氢欧山芹素、当归醇A、当归醇G、二氢欧山芹醇乙酸酯、蛇床子素、二氢欧山芹醇当归酸酯6个成分的含量。结果 15批独活饮片相似度在0.858~0.986，共标定了21个共有峰，指认出7个色谱峰，分别为二氢欧山芹素、当归醇A、当归醇G、二氢欧山芹醇乙酸酯、蛇床子素、异欧前胡素、二氢欧山芹醇当归酸酯，其中二氢欧山芹素、当归醇A、当归醇G、二氢欧山芹醇乙酸酯、蛇床子素、二氢欧山芹醇当归酸酯6种成分的含量分别为0.013%~0.051%、0.553%~0.866%、0.222%~0.396%、0.032%~0.259%、0.327%~1.229%、0.108%~0.473%。聚类分析将15批饮片分为3类，PLS-DA法标记出饮片中的8个差异性成分。结论 建立的指纹图谱及多成分定量分析方法稳定可靠，能为独活饮片质量标准的建立提供理论指导。Abstract: OBJECTIVE To establish the HPLC fingerprint and simultaneous method for the determination of 6 components for processed pieces and quality control of Angelica Pubescens Radix pieces . METHODS Fingerprints of 15 batches of Angelica Pubescens Radix pieces were determined by HPLC-PDA， the control fingerprint was established. Combined with cluster analysis and partial least square discriminant analysis， the quality of 15 batches of Angelica Pubescens Radix pieces was analyzed. At the same time， the contents of columbianetin， angelol A， angelol G， columbianetin acetate， osthole， isoimperatorin and columbianadin were determined. RESULTS The similarity of 15 batches of Angelica Pubescens Radix pieces was 0.858-0.986， and 21 common peaks were identified. Columbianetin， angelol A， angelol G， columbianetin acetate， osthole， isoimperatorin and columbianadin were identified， among which columbianetin， angelol A， angelol G， columbianetin acetate， osthole， isoimperatorin and columbianadin were quantitative analyzed. Their contents were 0.013%-0.051%， 0.553%-0.866%， 0.222%-0.396%， 0.032%-0.259%， 0.327%-1.229%， 0.108%-0.473%， respectively. Cluster analysis divides 15 batches of decoction pieces into three categories， and partial least squares discriminant analysis marks 8 different components in the decoction pieces. CONCLUSION The fingerprint and multi-component quantitative analysis method established in this experiment are stable and reliable， which can provide theoretical guidance for the establishment of quality standards of Angelica Pubescens Radix pieces.
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