Abstract: OBJECTIVE To investigate the mechanism of Xiangshenke formula in regulating the proliferation， migration and EMT of human gastric carcinoma cells SGC-7901. METHODS SGC-7901 cells were cultured in vitro， and then different concentrations of Xiangshenke formula containing serum were added into the cells. Wound healing test and transwell chamber assay were used to observe the SGC-7901 cell migration and invasion. MTT assay was used to investigate the SGC-7901 cell proliferation. Western blot was used to detect the expression levels of N-cadherin， Vimentin， E-cadherin， p-GSK3β/GSK3β and β-Catenin. RESULTS Xiangshenke formula effectively inhibited the migration and invasion of SGC-7901 cells， suppressed the cell proliferation， down-regulated the expression of N-cadherin， Vimentin， p-GSK3β/GSK3β and β-Catenin， and up-regulated the expression of E-cadherin. CONCLUSION Xiangshenke formula can inhibit the SGC-7901 cell epithelial-mesenchymal transition by regulating the GSK3β/β-Catenin signaling pathway， and suppress their migrtaion and invasion ability. It plays an important role in adjuvant therapy after gastric cancer surgery.