当归芍药散对控制性超促排卵致小鼠胎盘微绒毛膜葡萄糖转运体表达下调的保护作用

Protection of Danggui Shaoyao San on the Down-Regulated Glucose Transporter in Mice Placental Microvillous Membrane by Controlled Ovarian Hyperstimulation

    摘要
  • 摘要: 目的 观察当归芍药散对控制性超促排卵（COH）致小鼠胎盘微绒毛膜（MVM）葡萄糖转运体1（GLUT1）蛋白表达下调的影响。方法 将IVF囊胚分别移植入自然交配假孕鼠，超促排假孕鼠。自然交配受体组以自然交配假孕鼠为囊胚受体，超促排受体组以超促排假孕鼠为囊胚受体，以超促排假孕鼠为囊胚受体并于E3.5 d给予当归山药散治疗为当归芍药散治疗组。E18.5 d检测胚胎、胎盘质量及胎盘迷路层、连接层面积。qPCR检测胎盘Igfbp1、Igf1、Glut1 mRNA水平，ELISA法检测胎盘中IGFBP1，IGF1蛋白水平，Western blot检测胎盘、MVM中GLUT1蛋白水平。结果 超促排受体组较自然交配受体组E18.5 d胚胎、胎盘质量显著降低(P<0.01)，胎盘Igf1、Glut1 mRNA水平显著降低（P<0.01），胎盘、MVM中GLUT1蛋白水平显著降低(P<0.01)，胎盘Igfbp1 mRNA水平及IGFBP1蛋白水平显著增高（P<0.01）。当归芍药散组较超促排受体组E18.5 d胚胎、胎盘质量显著增高（P<0.01），胎盘Igf1、Glut1 mRNA水平显著增高（P<0.05），胎盘、MVM中GLUT1蛋白水平显著增高（P<0.05），胎盘Igfbp1 mRNA水平及IGFBP1蛋白水平显著降低（P<0.05）。结论 当归芍药散改善控制性超促排卵小鼠胚胎生长，上调控制性超促排卵小鼠MVM中GLUT1蛋白表达与抑制胎盘IGFBP1蛋白表达，促进胎盘IGF1蛋白表达有关。

     

    Abstract: OBJECTIVE To assess the protection of Danggui Shaoyao San on the down-regulated glucose transporter in mouse placental microvillous membrane (MVM) by controlled ovarian hyperstimulation. METHODS IVF blastocysts were transferred to non-stimulated or superovulated pseudopregnant recipient mice. The non-stimulated pseudopregnant recipient mice were non-stimulated recipient mice group. The superovulated pseudopregnant recipient mice were superovulated recipient mice group. The superovulated pseudopregnant recipient mice which received Danggui Shaoyao San treatment were Danggui Shaoyao San treatment group. At E18.5 d， the fetal weight and placental weight were examined. The mRNA expressions of Igfbp1， Igf1and Glut1 in placenta were determined by qPCR. The IGFBP1 and IGF1 protein levels in placental homogenates were determined by ELISA. The GLUT1 protein levels in placental homogenates and MVM were detected by Western blotting. RESULTS At E18.5 d， the mean weights of fetal and placental (P<0.01)， the mRNA for Igf1、Glut1 in placenta (P<0.01)， and GLUT1 protein levels of placental homogenates and MVM (P<0.01) in superovulated recipient mice group were significantly lower compared with non-stimulated recipient mice group. The mRNA for Igfbp1 in placenta of superovulated recipient mice group was significantly higher compared with non-stimulated recipient mice group (P<0.01). Meanwhile， the mean weights of fetal and placenta (P<0.01) ， the mRNA for Igf1， Glut1 in placenta (P<0.05)， and GLUT1 protein levels of placental homogenates and MVM (P<0.05) in Danggui Shaoyao San treatment group were significantly higher compared with those in superovulated recipient mice group. The mRNA for Igfbp1 in placenta of Danggui Shaoyao San treatment group was significantly lower compared with that in superovulated recipient mice group (P<0.05). CONCLUSION Danggui Shaoyao San plays protective effect on the down-regulated glucose transporter in mouse placental MVM by controlled ovarian hyperstimulation. The protection of Danggui Shaoyao San is associated with down-regulated IGFBP1 protein expression and up-regulated IGF1 protein expression in placenta.

     

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