Abstract: OBJECTIVE To find out herbs with significant differences in using frequency of prescriptions in the treatment of premature menstruation and delayed menstruation， and to determine their differences. METHODS Sixty five premature menstruation cases and 367 delayed menstruation were selected. Chi-square test and logistic regression were used to analyze the differences in herbs use between premature menstruation and delayed menstruation prescriptions. RESULTS There were 7 kinds of used herbs were significantly different between premature menstruation and delayed menstruation prescriptions， and they were jin yin hua (Flos Lonicerae Japonicae)， huang bai (Cortex Phellodendri Chinensis)， pu gong ying (Herba Taraxaci)， han lian cao (Herba Ecliptae)， ou jie (Nodus Nelumbinis Rhizomatis)， chang pu (Rhizoma Acori Graminei) and dang gui (Radix Angelicae Sinensis). In addition to dang gui (Radix Angelicae Sinensis)， the rest herbs were higher frequently used in premature menstruation than that in the delayed menstruation， from high to low according to the difference， they were han lian cao (Herba Ecliptae)， ou jie (Nodus Nelumbinis Rhizomatis)， pu gong ying (Herba Taraxaci)， chang pu (Rhizoma Acori Graminei)， jin yin hua (Flos Lonicerae Japonicae) and huang bai (Cortex Phellodendri Chinensis). CONCLUSIONS The herbs use difference between premature menstruation and delayed menstruation could be determined through chi-square test. Then， logistic regression can be used to analyze the difference degree， which can make the difference more specific， and provide reference for clinical herb use for premature menstruation and delayed menstruation.