Abstract: OBJECTIVE The protective effect of Guilu Erxian Glue on dexamethasone induced bone marrow mesenchymal stem cells injury. METHODS Bone marrow mesenchymal stem cells were obtained from SD rats and divided into four groups: normal group， dexamethasone (10-8) group， low dose and high dose of Guilu Erxian Glue groups. The activity of bone marrow mesenchymal stem cells was measured by MTT assay， superoxide dismutase (SOD)， malondialdehyde (MDA)， interleukin-6 (IL-6)， IL-1β， tumor necrosis factor a (TNF-α) were measured. The expression of Nrf-2/HO-1/NF-κB signal proteins was detected by Western blot. RESULTS Guilu Erxian Glue groups can significantly improve cell vitality， increase SOD level， reduce the level of MDA， reduce the level of inflammation factor， increase the expression of Protein， Nrf-2 and HO-1 proteins， and reduce the expression of NF-κB signal proteins. CONCLUSION Guilu Erxian Glue can inhibit the injury of bone marrow mesenchymal stem cells by dexamethasone， and the role is related to the regulation of Nrf-2/HO-1/NF-κB signal.