Abstract: OBJECTIVE To evaluate the protective effects of Cyclovirobuxine D on myocardial ischemic rats by the method of Micro-PET scanning technology. METHODS All male rats were randomly divided into sham group， model group， digoxin group and Cyclovirobuxine D group. The myocardial ischemic models were established in all groups except sham group. The imaging agent 18FFDG was injected intravenously into tail in rats on the first day of the model and 7th day during medication. The Standard Uptake Value(SUV) of the heart was measured by the method of Micro-PET scanning technology. The changes of myocardial infarction rate were evaluated by Volume of Interest (VOI) sketch. RESULTS The levels of SUV of the heart were improved and the myocardial infarction rates were reduced in digoxin group and Cyclovirobuxine D group(P<0.05~0.01)， compared with the model group. CONCLUSION Cyclovirobuxine D has protective effects on myocardial ischemic rats， which can be concluded by the Micro-PET imaging technology in living conditions of rats.