HPLC同时测定消癥丸中柴胡皂苷a、芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚和橙皮苷

Study on Simultaneous Determination of Saikosaponin a， Aloe-emodin， Rhein， Emodin， Chrysophanol， Emodin and Hesperidin in Xiaozheng Pills with HPLC

摘要: 目的建立HPLC法同时测定消癥丸柴胡皂苷a、芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚和橙皮苷7个成分的方法。方法采用HPLC，Waters Xbridge C₁₈柱（4.6 mm×250 mm，5 μm）；以乙腈（A）-0.3%磷酸水溶液（B）为流动相进行梯度洗脱，流速0.8 mL/min，检测波长λ₁=210 nm，λ₂₌300 nm，柱温40 ℃。结果消癥丸中7种成分均能达到基线分离并进行了含量测定。结论本法准确、可靠、重复性好，可为消癥丸的质量控制提供更全面的依据。

Abstract: OBJECTIVE To apply HPLC for simultaneous determination of Saikosaponin a， aloe-emodin， rhein， emodin， chrysophanol， emodin and hesperidin in Xiaozheng pills. METHODS An HPLC method was established. Waters Xbridge C₁₈ column（4.6 mm×250 mm，5 μm）was used as analytical column with a mobile phase consisting of acetonitrile (A)-0.3% phosphoric acid (B). The flow rate was 0.8 mL/min， detection wavelengths λ₁ = 210 nm and λ₂ = 300 nm and the column temperature 40 ℃. RESULTS The seven ingredients all reached baseline separation and were determined. CONCLUSION HPLC method is accurate， reliable and repeatable， which can provide a more comprehensive basis for quality control of Xiaozheng pills.