Abstract: OBJECTIVE To observe effect of Xiao'er Jinning Oral Liquid on bacterium and virus in vivo and vitro. METHODS The antibacterial effect in vitro of Xiao'er Jinning Oral Liquid on common 12 kinds of pathogenic bacteria in clinic was observed through test tube methods while antibacterial affected in vivo by infecting mice with Staphylococcus aureus and Streptococcus pneumoniae. The antiviral effect in vitro was observed through cytopenic method induced by influenza A and influenza B while antiviral effect in vivo through mouse models with respiratory infection by influenza virus and Sendai virus. RESULTS Xiao'er Jinning Oral Liquid had an inhibitory effect on the 9 kinds of bacteria， influenza A， influenza B in different degrees， especially more for Staphylococcus aureus and influenza A， reduced mortality rates of mice infected by Staphylococcus aureus，influenza virus and Sendai virus with varying degrees， prolonged the survival time， decreased lung index of infected mice and diminished pulmonary pathological lesion. CONCLUSION Xiao'er Jinning Oral Liquid has some antibacterial and antiviral effect.